摘要
采用聚丙烯酰胺凝胶圆盘电泳分析方法，研究了幼年鼠和成年鼠睾丸乳酸脱氢酶（ＬＤＨ）同工酶与超氧化物歧化酶（ＳＯＤ）的酶带变化。观察结果表明，成年鼠睾丸的凝胶电泳胶条，多数显现六种ＬＤＨ同功酶带，其中ＬＤＨ５和ＬＤＨ４占优势，ＬＤＨ１、ＬＤＨ２、ＬＤＨ３显带较弱，在正极端ＬＤＨ的下面是第６条酶带，相当于Ｃ亚单位（ＬＤＨｃ）的Ｃ４。幼年鼠睾丸的凝胶电泳胶条，只显现ＬＤＨ１和ＬＤＨ２两条同功酶带，以ＬＤＨ１占优势。酶谱的变化说明，幼年鼠睾丸的ＬＤＨ同功酶是以Ｂ基因表达为主，成年鼠睾丸的ＬＤＨ同功酶则转变成以Ａ基因表达为主。ＬＤＨｃ酶带的出现，表明成年鼠睾丸还有Ｃ基因的表达。睾丸ＳＯＤ活性带变化提示，成年鼠睾丸的ＳＯＤ带活性强于幼年鼠的活性，这与成年鼠睾丸的ＳＯＤ不断地清除超氧化物自由基对精子发生过程的损伤有关。
A study of isoenzyme band changes on LDH and SOD in testis of juvenlie and adult mouse were cacrried out using acrylamide gel disc electrophoresis. The redults indicated that testis of adult mice showed six kinds of bands with obvious LDH 5 and LDH 4 bands. The sixth isoenzyme band was under of LDH 1 whilh from C sub unit LDH 4 of C 4. The testis of juveniles showed only LDH 1 and LDH 2 bands with dominant LDH 1 in the electrophoritic band. Corresponding to the a change of isozyme patterms indicated that LDH isozyme of juvenite testis showed mainly expression of B gene, LDH isozyme of adult showed mainly expression of A gene, sixth LDH isozyme band of adult testis indicated expression of C gene. The SOD activity band of testis suggested that the SOD activity of adult mice was stronger than that of childhood mice. This is possibly related to the testis of adult SOD eradicates constantly the injury to of sperms by free redical of superoxide.
出处
《动物学杂志》
CAS
CSCD
北大核心
1998年第1期40-43,共4页
Chinese Journal of Zoology
基金
天津市高等教育委员会资助
关键词
睾丸
乳酸氢酶
同功酶
SOD
成年人
幼年鼠
Mouse Testis PAGE Lactic dehydrogenase isenzyme Superoxide dismutase
