摘要
本文采用聚丙烯酰胺凝胶电泳方法,对中国林蛙(Rana chensinensis)早期胚胎发育过程中(受精后0—512h)乳酸脱氨酶(LDH)同工酶和酯酶(EST)同工酶进行了研究。结果表明:1.LDH同工酶在受精卵及早期胚胎发育过程中一直存在,并且在胚胎发育的不同时期差异显著。LDH_1同工酶在胚胎发育的各个阶段都占绝对优势,而LDH_5活性从尾芽期开始增强,LDH_2、LDH_3、LDH_43种同工酶的活性较低;同时也发现LDH_1、LDH_3、LDH_4同工酶各亚带也具有发育阶段的特异性。2.EST同工酶在开口期才开始出现。
This paper reports the analysis of lactate dehydrogenase ( LDH) and Esterase isoenzyme (EST) during Embryonic development of Rana chensinensis and optical density scanning. The results show that there are different type LDH isoenzymes in each stages, LDH, keeps higher activity during whole developing process, and the relative activities of LDH2, LDH3 and LDH5 are lower. The relative activities of LDH4 gradually increases from Tail bud stage. In addition, LDHP LDH3, LDH4 subbands specificity in the embryonic development has been discovered. The EST are observed from Mouth open stage. It is clear these ontogenetic changes are reflected the action of the differential genes (Ldh-A & Ldh-B) .
关键词
中国林蛙
胚胎发育
同功酶
蛙科
Pana chensinensis, Early embryonic development, Isoenzymes, Electrophoresis
