摘要
目的:探讨内毒素与博莱霉素所致肺损伤的病理特征及肺泡Ⅱ型上皮细胞形态和功能的变化。方法:将60只SD大鼠随机分为内毒素组、博莱霉素组、对照组,内毒素组经尾静脉注射内毒素5 mg/kg,博莱霉素组给予气管内注入博莱霉素5 mg/kg,对照组气管内及尾静脉注入等体积的生理盐水。分别于给药后的第3,7,14,28天处死5只。肺组织HE染色及Masson染色行病理组织学检查及电镜观察其超微结构的改变。结果:内毒素组早期肺泡炎较重,短期内恢复正常,肺泡Ⅱ型上皮细胞主要表现为线粒体的水肿,板层小体的脱颗粒,增生不明显。博莱霉素组早期肺泡炎,随之出现纤维化,并逐渐加重。Ⅱ型上皮细胞变性坏死,线粒体破坏。结论:内毒素与博莱霉素虽均可导致肺损伤,前者恢复正常,后者演变成肺纤维化,可能与肺泡Ⅱ型上皮细胞损伤的程度有关。
To study the pathologic changes in lung injury and pulmonary fibrosis induced by endotoxin and bleomycin. Methods： Sixty rats were separated into three groups at random as endotoxin group, bleomycin group, and control group according to the intratracheal or via caudal vein injection of endotoxin, hleomycin, and normal saline respectively. Five rats from every group were sacrificed on the 3rd, 7th, 14th, and 28th day to observe the pathologic changes in lung tissue by using transmission electronic microscope. Results： In endotoxin group, the degree of alveolitis was severe. Alveolar epithelium cells type Ⅱ （AT Ⅱ s） showed edema in the mito- chondrion and degranulation of osmiophilic lamellar body, hut hyperplasia was not obvious. In bleomycin group alveolitis was observed that happened slowly and developed into progressive pul- monary fibrosis, and AT Ⅱ s were necrotic gradually. Deposition of collagen fibres around the mesynchemal cells were also present. Conclusion： Both endotoxin and bleomycin could cause pulmonary injury, and the former could recover, but the later would develop into progressive pulmonary fibrosis. This may be related to the degree of ATⅡ injury.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2009年第3期326-329,共4页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:30670938)
关键词
肺损伤
肺纤维化
肺泡上皮细胞
内毒素
博莱霉素
Lung Injury
Pulmonary Fibrosis
Alveolar Epithelium Cell
Endotoxin
Bleo-mycin
