摘要
将单克隆抗体与溶解性受酸度或温度调节的高分子共价连接．研究了共聚物的溶解性可调节特征，建立了以溶解性可调节高分子为载体酶免疫分析方法，对血清样品中ＨＢｓＡｇ进行了检测，灵敏度０５μｇ／Ｌ，对４３例样品检验的结果与ＥＬＩＳＡ方法相符．
Solubleinsoluble forms of monoclonal antibody, which can be rendered either soluble or insoluble by simple adjusting the temperature or pH, have been prepared by covalently coupling antibody to poly(Nisopropylacrylamide) or to copolymer of methacrylic acid and acrylamide. These carriers were used in enzyme immunoassay instead of polystyrenes. Some advantages of both heterogeneous and homogeneous immunoassays were achieved. HBsAg was assayed to examine the sensitivity of these methods. A significant difference from the negative serum was obtained for 05 μg/L. 43 serum samples were assayed, and there was a good correlation between these methods and ELISA.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第1期79-81,共3页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
