摘要
在红豆杉培养红胞中，桔青霉诱导子促进紫杉醇的合成。将培养６—７ｄ的桔青霉菌丝体的粗提物，以５０μｇ碳水化合物／ｍｌ培养液的浓度加入到处于指数生长期末期的红豆杉培养细胞中，诱导子促进紫杉醇合成的作用最大。高压灭菌处理２０—９０ｍｉｎ，不影响诱导子的活性。
The Penicillum citrinum elicitor was extraxted after autoclaveing a 6～7-day old culture of P. citrinum which could promote taxol biosynthsis in suspension cell cultures of Taxus chinensis. The optimum elicitor cocentration was found to be 50μg carbohydrate/ml medium of T. chinensis cell cultures. T. chinensis cells showed strongest response to the elicitor during the growth phase(20d). The time of autoclaving(20～90min)did not affect the elicitor's activity.
出处
《植物研究》
CAS
CSCD
北大核心
1998年第1期78-82,共5页
Bulletin of Botanical Research
关键词
桔青霉
诱导子
紫杉醇
细胞培养
红豆杉
Penicillum citrinum
Elicitor
Taxol
Cell culture
Taxus chinensis
