摘要
目的：探讨体外循环是否影响多形核白细胞（ＰＭＮＬ）表面粘附分子ＣＤ１１ｂ和ＣＤ１８水平对合并肺感染的诊断。方法：采用免疫荧光流式细胞技术，观察４组患者体外循环术后ＰＭＮＬ表面粘附分子ＣＤ１１ｂ和ＣＤ１８的表达水平。Ⅰ组：１０例手术前患者；Ⅱ组：体外循环术后当天回ＩＣＵ的患者；Ⅲ组：１０例体外循环术后１周内合并肺感染患者；Ⅳ组：１０例体外循环术后１周内恢复良好患者。结果：体外循环可使患者ＰＭＮＬ表面ＣＤ１１ｂ和ＣＤ１８的表达上调（Ｐ＜００１）；体外循环术后合并肺感染患者ＰＭＮＬ表面ＣＤ１１ｂ和ＣＤ１８的表达水平则再次上调（Ｐ＜００１），而体外循环术后恢复良好的患者ＰＭＮＬ表面ＣＤ１１ｂ和ＣＤ１８的表达水平仍处于低水平。结论：体外循环不影响以ＰＭＮＬ表面ＣＤ１１ｂ和ＣＤ１８水平对合并肺感染的诊断。
Objective:To investigate the clinical significance of CD 11 b and CD 18 for the diagnosis of pulmonary infection after cardiopulmonary bypass(CPB) could be influenced by the CPB technique. Method:The immunofluorescence flow cytometry was used to determine the expression of CD 11 b and CD 18 on the surface of polymorphnuclear leucocytes(PMNL) in 4 groups of patients.Ⅰ:10 preoperative patients.Ⅱ:10 ICU patients just underwent CPB.Ⅲ:10 patients complicated with pulmonary infection in one week after CPB.Ⅳ:10 wellrecovered patients underwent CPB in one week.Results:CPB can induce the upregulation of CD 11 b and CD 18 expression on PMNL in patients just underwent CPB(P<001).The expressions of CD 11 b and CD 18 on the surface of PMNL was upregulated once again in patients after they complicated with pulmonary infection(P<001),but the expression of CD 11 b and CD 18 was maintained low level in wellrecovered patients underwent CPB.Conclusion:The value of CD 11 b and CD 18 for diagnosis of pulmonary infection after CPB is not influenced by the CPB technique.
出处
《中日友好医院学报》
1998年第1期29-3,共1页
Journal of China-Japan Friendship Hospital
关键词
体外循环
多形核白细胞
CD11B
CD18
肺感染
extracorporeal circulation
lung diseases
polymorphnuclear leucocyte
CD 11 b/CD 18
