摘要
目的研究氧化ＬＤＬ和抗氧化剂对人脐静脉内皮细胞一氧化氮的影响。方法用一氧化氮试剂盒测试培养的人脐静脉内皮细胞一氧化氮含量。结果氧化ＬＤＬ对培养的内皮细胞一氧化氮产生有明显抑制作用（Ｐ＜０．０１），并且具有浓度和时间依赖效应。抗氧化剂ＶｉｔＥ可明显减弱氧化的ＬＤＬ对内皮细胞的损伤，与ＯＸ－ＬＤＬ组相比一氧化氮含量增加２１％～６０％（Ｐ＜０．０１）。结论氧化ＬＤＬ可以明显抑制人脐静脉内皮细胞一氧化氮的合成，抗氧化剂ＶｉｔＥ有明显的保护作用。
Aim To study the effect of OXLDL and antioxidant on NO production in cultured endothelial cells of human umbilical vein . Methods The level of NO was measured in cultured endothelial cells in human umbilical vein by NO kits. Results OXLDL significantly decrease the level of NO in a dose and timedependent manner in cultured endothelial cells(P<001). Antioxidant VitE can significantly decrease the injury of OXLDL in cultured endothelial cells. NO is increased 21%￣60%, compared with that in OXLDL group(P<001). Conclusion OXLDL significantly decrease the NO systhesis, antioxidant Vit E can significantly protect endothelial cells from the injury of OXLDL
出处
《高血压杂志》
CSCD
1998年第1期19-21,共3页
Chinese Journal of Hypertension
关键词
低密度脂蛋白
内皮细胞
抗氧化剂
心血管疾病
oxidized low density lipoproteins
endothelial cells
nitric oxide
antioxidant
