摘要
目的：探讨成釉细胞离体培养方法及其特性。方法：采用贴壁培养法培养大鼠成釉细胞，用倒置显微镜观察细胞形态，用免疫组化检测细胞合成釉原蛋白的情况，用电镜观察成釉细胞离体培养时的形态。结果：大鼠成釉上皮细胞经消化酶解后原代培养于明胶包被的培养皿上，细胞贴壁良好，仅有少量成纤维细胞。在培养细胞生长一定时间后，主要为上皮型细胞成片生长，在细胞传至Ｆ２代时仍能分泌釉原蛋白。传至Ｆ５代时，细胞逐渐转变为长梭形，类似成纤维细胞。扫描电镜可见细胞突起，并有基质分泌。结论：用明胶作基质，对大鼠成釉细胞作离体培养，可以较长时间地维持其细胞特性。
Aim: To develope a culture model for the rat ameloblast and it's biological study. Methods: The rat ameroblasts were separated by collagenase and trypsin, and cultured on a substrase of gelatin. The cultured ameloblasts were examined by SEM and immunohistochemically for it's expression of amelogenins. Results: The inner epithelia cell layer were primarily cultured on gelatin coated culture dishes having a tipical epithelium cell characteristics.Expression of amelogenin was observed even when passaged to F2(about 4ws). On some dishes,the cells turn to be fibroblast-like cells when passaged to F5. These cells enlonged and secreted extracellular matrix when examined by SEM. Conclusion: Ameloblast could be cultured for a longer time maitainning it's claracteristics when gelatin was used as substrase.
出处
《牙体牙髓牙周病学杂志》
CAS
1998年第1期46-48,共3页
Chinese Journal of Conservative Dentistry
