摘要
为建立并优化适于香蕉(Musaspp.)SRAP分析的扩增体系,对影响香蕉SRAP反应的dNTP、Mg2+、模板DNA、引物浓度和Taq酶用量等因素进行优化。确定的优化扩增体系为Mg2+2.5mmol.L-1,dNTP250μmol.L-1,Taq酶1.0U,引物0.5μmol.L-1,模板DNA20ng,10×PCRbuffer2.5μL,在此条件下SRAP扩增香蕉基因组DNA条带清晰,多态性丰富。该体系在29个香蕉基因组中获得较好的扩增结果,可望在香蕉植物起源和进化研究中应用。
In order to establish and optimize the SRAP molecular marker system in Musa spp. , the concentrations of Mg^2＋ , dNTPs, Taq DNA polymerase, primers which affect the SRAP-PCR reactions were optimized. The optimum system was as follows ： Mg^2＋ 2.5 mmol· mL ^- 1, dNTPs 250 μmol·L^-1, Taq DNA polymerase 1.0 U, primer 0.5 μmol·L^-1 , template DNA 20 ng, 10 ×PCR buffer 2.5μL. The total volume of reaction was 25 μL. Amplications were carriyed out on 29 banana cuhivars genome using this optimum system. The results showed that the system was steady and reliable and would be helpful to study origin and evolution of Musa spp.
出处
《植物研究》
CAS
CSCD
北大核心
2009年第3期352-356,共5页
Bulletin of Botanical Research
基金
海南省自然基金项目(批准号:30823)
中央级公益性科研院所基本科研业务费专项基金(项目编号:PZS028)
中国热带农业科学院引进人才科研启动项目(项目编号:Pzsb0801)资助
