摘要
以唐古特白刺(Nitraria tangutorum Bobr.)愈伤组织为材料,研究一氧化氮(NO)供体硝普钠(SNP)处理对其膜脂过氧化及抗氧化酶活性的影响。激光共聚焦扫描显微镜技术检测结果显示,白刺愈伤组织在SNP处理下细胞中NO含量显著增加,而N-硝基-L-精氨酸(L-NNA)处理使细胞中NO水平降低。低浓度SNP(10和25μmol.L-1)处理后,白刺愈伤组织中丙二醛(MDA)含量显著减少,而高浓度SNP(100μmol.L-1)和L-NNA(0.5mmol.L-1)处理后MDA含量明显高于对照。与对照比,SNP处理诱导白刺愈伤组织可溶性蛋白含量升高,却使脯氨酸含量减少。白刺愈伤组织在不同浓度SNP处理下过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性呈增加的变化趋势,低浓度SNP处理使过氧化物酶(POD)活性减弱,而50μmol.L-1SNP处理后POD活性约增加为对照的119%。结果提示,外源NO可促进白刺愈伤组织可溶性蛋白的合成,诱导抗氧化酶活性升高,增强了白刺愈伤组织的抗氧化能力,从而表现出对细胞的保护作用。
The experiments were conducted with the callus of Nitraria tangutorum Bobr. for studying the changes of lipid peroxidation and antioxidase activity under treatment with SNP（ Sodium Nitroprusside）, as a NO donor. Investigation of NO content using laser scanning confocal microscope （LSCM） technology showed that there was a significant increase of NO level in the callus of N. tangutorum Bobr. in response to SNP treatment, but a decrease induced by L-NNA. Remarkable reduce of MDA content was observed in N. tangutorum Bobr. callus treated with low SNP concentration, but 100μmol·L^-1 SNP or 0.5 mmol · L-1 L-NNA induced enhanced MDA content in comparison with the control. Compared to the control, SNP treatment induced increase in the amount of soluble protein, whereas decrease in proline content the callus of N. tangutorum Bobr.. SNP treatment resulted in increasing CAT and APX activities in a concentration-dependent manner. In addition, treatment with low SNP concentration reduced POD activity, but POD activity increased to 119% of contrast in callus treated with 50 μmol·L^-1 SNP. In conclusion, the results testified that exogenous NO induced synthesis of soluble protein, enhanced antioxidant capacity and antioxidase activity in the callus of N. tangutorum Bobr. , suggesting that the protective effect of NO to N. tangutorum Bobr. cell was proved.
出处
《植物研究》
CAS
CSCD
北大核心
2009年第3期303-307,共5页
Bulletin of Botanical Research
基金
甘肃省中青年科技研究基金项目(0806RJYA005)
西北师范大学科研骨干培育项目(nwnu-kjcxgc-03-44)
西北师范大学学生学术资助金项目资助
关键词
唐古特白刺
愈伤组织
一氧化氮
膜脂过氧化
抗氧化酶
Nitraria tangutorum Bobr.
callus
nitric oxide
lipid peroxidation
antioxidant enzyme
