摘要
来源于人流组织的蜕膜和绒毛膜,异种接种于发育8d的鸡胚绒毛尿囊膜(ChickenChorioallantoic Membrane,CAM)上,进行在体培养观察10d.将378块绒毛膜和808块蜕膜组织分别种植于69和122个鸡胚,鸡胚胎存活率分别为86.96%和98.36%(P<0.01),种植组织成活率分别为93.62%和93.81%;种植组织于在体培养过程中,CAM表面产生血管增生及出血现象,出血率分别为41.67%和20.37%(P<0.05).两种组织种植后均无异种排斥反应和炎症反应发生.建立CAM在体培养模型,简便快速,观察方便,可同时进行大样本实验,是一种很好的实验手段.
Human decidua and trophoblast villi derived from early pregnancy were transplanted onto Chicken Choriollantoic membrane (CAM) and were observed and survived for 10 days. Total 69 eggs for transplanting decidua and 122 eggs for trophoblast villi were used. Survival rate of the eggs for the two groups were 86. 96% and 98. 36% respectively (P<0. 01). Total of 378 pieces of decidual tissue and 808 pieces of trophoblast villi were transplanted. Survival rate of the tissue for the two groups were 93. 62% and 93. 81% respectively. After transplantation,bleeding occurred on the surface of the CAM in some cases,being 41. 67% and 20. 37% for the two types of tissue respectively. No rejection and inflammation occur after transplantation. The xenograft on CAM provided a convenient and rapid means for in vivo transplantation and observation which allowsa large amount of sample to be studied at one time.
出处
《生殖与避孕》
CAS
CSCD
北大核心
1998年第1期22-26,共5页
Reproduction and Contraception
关键词
血和生成
动物模型
蜕膜
绒毛膜
尿囊膜
种植
Chicken Embryo, Angiogenesis, Animal model, Decidua, Chorionicvilli
