摘要
用分批培养的方法研究了经ＣＨ２Ｃｌ２或ＣＨ３ＯＨ驯化的假单胞杆菌ＤＭＩ对ＣＨ３ＯＨ和 ＣＨ２Ｃｌ２竞争性利用的动力学．ＣＨ２Ｃｌ２的降解过程是严重底物抑制过程，ＣＨ２Ｃｌ２和ＣＨ３ＯＨ 的同时利用有助于提高菌体的生长速率．两种底物共存时，甲醇的消耗占优势、同种底物比 的消耗速率均低于在单一碳源时的数值．高浓度甲醇的竞争作用不但抑制了ＣＨ２Ｃｌ２驯化的 ＤＭＩ对ＣＨ２Ｃｌ２的降解能力，而且抑制了ＣＨ３ＯＨ驯化菌脱卤活性的诱导过程．两种底物消 耗的相对速率取决于接种物的生物活性和底物的相互作用．
Competitive utilization of CH2Cl2 and CH3OH by the preacclimated pseudomonas sp. strain DMI was studied in batch cultures. The degradation of CH2Cl2 by DMI was a process with severe substrate inhibition .The sim simulta- neous utilization of CH2Cl2 and CH3OH prompted the bacteria to enhance their growth rate. When the two substrates coexisted, DMI showed a preference of degrading methanol over dichloromethane. The specific consumption rate of each substrate was lower than that when either was used as the sole carbon and energy source. The competition of methanol of high concenttatinn inhibited noly CH2Cl2 biode- gradation capability for the CH2Cl2- preacclimated DM1, but also the induction rate of pehalo -genation activity for the CH3 OH-Preacclimated one. The relative rate of consumption the two subtrates was dependent on the bioactivity of the inoculum and the interacion of substrates.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
1989年第4期114-119,共6页
Journal of Fuzhou University(Natural Science Edition)
关键词
二氯甲烷
甲醇
废水
假单胞杆菌
pseudomonas DMI, biodegradation, competitive utilization
