摘要
[目的]寻找快速获得整齐一致的虎舌红苗木的方法,为虎舌红的产业化生产提供理论与技术支撑。[方法]以虎舌红当年生枝条和带芽茎段为外植体进行组织培养试验。[结果]诱导虎舌红芽分化的最优激素组合是TDZ 0.5 mg/L+NAA 0.2 mg/L+AgNO33.0mg/L,其平均诱导率达94.56%;其次是TDZ 0.5 mg/L+NAA 0.4 mg/L+AgNO35.0 mg/L,其平均诱导率为89.18%;2号和3号培养基上的芽分化率最低,不到35%。诱导虎舌红芽增殖的最佳激素组合是6-BA 1.5 mg/L+NAA 0.5 mg/L,其平均增殖系数为5.14。IBA对虎舌红生根的影响比NAA大,培养基1/2 MS+IBA 0.3 mg/L+NAA 0.5 mg/L诱导虎舌红生根的效果较好,生根率达75.8%。虎舌红组培幼苗的移栽成活率达87.6%。[结论]诱导虎舌红芽分化的最佳培养基为1/2 MS+TDZ 0.5 mg/L+NAA 0.2 mg/L+AgNO33.0 mg/L,最佳增殖培养基为1/2 MS+6-BA 1.5 mg/L+NAA 0.5 mg/L,最佳生根培养基为1/2 MS+IBA 0.3 mg/L+NAA 0.5 mg/L。
[Objective] The aim of the study was to fmd a method of getting uniform Ardisia mamillata seedlings rapidly and provide theoretical and technical support for its industrial production. [Method] With current - year branches and stem sections with buds of A. mamillata as explants, the experiment on its tissue culture was performed. [Result] The optimum hormone combination for inducing the bud differentiation of A. mam/l/ata was TDZ 0.5 mg/L＋ NAA 0.2 mg/L＋ AgNO3 3.0 mg/L and its average induction rate was up to 94.56% ; TDZ 0.5 mg/L＋ NAA 0.4 mg/L＋ AgNO3 5.0 rag/ L was secondary and its average induction rate was 89.18%. The bud differentiation rate was lowest on media 2 and 3, which was less than 35%. The optimum hormone combination for inducing the bud nrahiplication of A. mamillata was 6-BA 1.5 mg/L ＋ NAA 0.5 mg/L and its average multiplication coefficient was 5.14. The effect of IAA on the rooting of A. mamillata was greater than that of NAA. The effect on inducing the rooting of A. mamillata of medium 1/2 MS ＋ IBA 0.3 mg/L＋ NAA 0.5 mg/L was better and its rooting rate was up to 75.8%. The survival rate of transplanted A. mamillata seedlings from tissue culture was up to 87.6%. [Conclusion] The optimum medium for inducing the bud differentiation of A. mamillata was 1/2 MS＋ TDZ 0.5 mg/L＋ NAA 0.2 mg/L＋ AgNO3 3.0mg/L, that for inducing its bud multiplication was 1/2 MS ＋ 6-BA 1.5 mg/L＋ NAA 0.5 mg/L and that for inducing its rooting was 1/2 MS ＋ IBA 0.3 mg/L＋ NAA 0.5 mg/L.
出处
《安徽农业科学》
CAS
北大核心
2009年第13期5875-5876,共2页
Journal of Anhui Agricultural Sciences
基金
江西省教育厅科技项目(GJJ09603)
关键词
虎舌红
组织培养
快速繁殖
Ardisia mamillata
Tissue culture
Rapid propagation
