摘要
目的:探讨可溶性人类白细胞抗原G(sHLA-G)与IVF临床妊娠率的关系。方法:收集IVF-ET患者体外单个培养胚胎的培养液,通过ELISA法检测培养液中sHLA-G的浓度,并计算相应移植胚胎的妊娠率。结果:在41名患者的84份胚胎培养液中,sHLA-G阳性率52%(44/84),阳性培养液中sHLA-G浓度为3.7￣16.6ng/ml;移植至少一个sHLA-G阳性胚胎的妊娠率为69%,移植胚胎全部为sHLA-G阴性的妊娠率为18%,两者相比有统计学差异(P<0.05)。结论:以sHLA-G阳性表达水平结合传统胚胎形态学评估方法,可以作为辅助选择移植胚胎的临床指标,预测IVF妊娠成功率。
Objective： To investigate the correlation between soluble HLA-G and clinical pregnancy rates. Methods： Patients of IVF-ET were selected, the culture supernatants from cultured individually embryo in vitro were collected, sHLA-G concentration was measured by ELISA assay kit and the pregnancy rate of embryo transfered was calculated. Results： In the 84 embryo culture supernatants from 41 patients, the positive rate of sHLA-G was 52% （44/84）, sHLA-G concentration in positive cultured supernatants was 3.7-16.6 ng/ml; when at least one transferred embryo was sHLA-G positive, pregnancy rate was 69%, and only embryos transferred that were negative for sHLA-G had a pregnancy rate of 18%, there was a significant difference （P〈0.05）. Conclusion： Addition of sHLA-G to traditional morphological criteria may be useful as a clinical tool for embryo selection and predicting pregnancy rate of IVF.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2009年第4期257-260,共4页
Reproduction and Contraception
基金
国家973项目,项目号:2007CB948102
