摘要
目的:探讨补阳还五汤对局灶性脑缺血大鼠脑内神经细胞黏附分子(NCAM)表达的影响。方法:采用大脑中动脉阻塞法复制脑缺血模型,动物随机分为正常组、假手术组、模型组和补阳还五汤组,各组分别给予不同处理,分别于12h,1,3,7,14d处死动物,采用免疫组织化学法观察NCAM的表达。结果:在正常脑内有少量NCAM阳性细胞,缺血后表达增加,于术后第3天达高峰,补阳还五汤组能显著提高NCAM的表达,在第7、14天模型组与补阳还五汤组差异有显著性(P<0.01)。结论:补阳还五汤增强脑缺血后NCAM的表达是其促进神经再生和功能恢复的可能机制之一。
Objective： To investigate the effect of Buyanghuanwu Decoction （BYHW） on expression of neural cell adhesion molecule （NCAM） in rat models of cerebral ischemia. Methods： Brain ischemia was induced by middle cerebral artery occlusion. The rats were randomly divided into four groups, including normal group, sham-operated group, model group and BYHW Decoction group. Rats in each group were given different treatments and killed at 12 h, 1, 3, 7 or 14 d after operation. NCAM expression were measured by immunohistochemical staining. Results： There were a few NCAM positive cells in normal brain, expression of neural cell adhesion molecule was enhanced immediately after ischemia and reached peak at 3 d after ischemia; BYHW Decoction group increased the number of NCAM positive cells. This increase was statistically significant at 7 and 14 d after ischemia and treatment （P〈0.01）. Conclusion： BYHW Decoction is capable of regulating expression of NCAM in ischemic brain, that may be a neurogenic mechanism of BYHW Decoction.
出处
《中国医药导报》
CAS
2009年第14期33-35,共3页
China Medical Herald
基金
国家自然科学基金(30873355
30472217
30300470)
教育部博士点基金(20060541007)
湖南省自然科学基金(06JJY2052)
湖南省教育厅科学基金(08A049)
关键词
脑缺血/中医药疗法
神经细胞黏附分子
补阳还五汤
Cerebral ischemic/TCM therapy
Neural cell adhesion molecule
Buyanghuanwu Decoction
