摘要
目的建立中成药雷公藤多甙片有效成分胶束毛细管电泳优化分离分析方法.方法在熔融硅胶毛细管(有效柱长57 cm,内径为75μm)柱上,施加20 kV电压,温度298 K,进样时间5 s,214 nm紫外检测,缓冲体系包括10 mmol.L-1硼砂中加入20 mmol.L-1十二烷基硫酸钠(SDS)作表面活性剂,用硼酸调节pH7.30.结果优化分离条件下有效成分可达基线分离,有良好的线性关系.结论该方法简便快速可靠,可用于雷公藤多甙片中有效成分的测定.
Objective To develop a HPCE method for optimization separation of active ingredients from Multi-glycosides Tripterygium wilfordii traditional Chinese medicine. Methods Measurements were carried out in a buffer solution containing 10 mmol·L^-1 and added to SDS （pH7.3） at 20KV running voltage applied to a 57cm×75um capillary. The detection wavelength was 214mn. The column temperature was 298K. Injection time was 5s. Results The active ingredients eached the baseline seperation and had a good linear relationship. Conclusion The method is simple, rapid and reliable. It can be used to control the quality of Multi-glycosides Tripterygiurn wilfordii.
出处
《河北北方学院学报（自然科学版）》
2009年第2期33-35,共3页
Journal of Hebei North University:Natural Science Edition
关键词
高效毛细管电泳
雷公藤多甙片
活性成分
HPCE
Multi-glycosides Tripterygium wilfordii tablets
active ingredients
