摘要
目的探讨传统免疫印迹法(IB)和线性免疫印迹法(LB)检测Sm抗体的结果差异。方法用IB和LB分别测定48例系统性红斑狼疮(SLE)和30例正常人血清中Sm抗体,评价两者的敏感性和特异性。结果IB和LB法检测SLE患者血清中Sm抗体的敏感性分别是47.9%和37.5%;两者的特异性分别是100.0%和96.7%。结论IB法检测SLE患者Sm抗体的敏感性高于LB法(P<0.05);而两者的特异性差异无统计学意义(P>0.05)。
Objective To study comparatively the difference between immunoblotting（IB） and line-immunoblotting assay（LB） in the determination of Sin antibody. Methods Sera Sm antibody of 48 patients with systemic lupus erythematosus（SLE） and 30 healthy controls was detected by IB and LB, and analyzed respectively. The sensitivity and specificity of the assays were evaluated. Results The sensitivity of IB and LB assays were 47.9% and 37.5% respectively. The specificity of IB and LB assays were respectively 100.0% and 96.7%. Conclusion Our research has showed that IB assay is more sensitivily than LB（ P 〈 0. 05 ）, but the specificity of two assays have no difference（ P 〉0.05 ）.
出处
《中华全科医学》
2009年第5期532-532,540,共2页
Chinese Journal of General Practice
关键词
免疫印迹
系统性红斑狼疮
SM抗体
Immunoblotting
Systemic lupus erythematosus
Sm antibody
