摘要
目的:探讨乳腺癌组织中类泛素化蛋白酶1(SENP1)和原癌基因CerBb-2蛋白的表达,并分析两者与乳腺癌病理因素的关系。方法:采用ABC免疫组化染色法,检测乳腺癌及乳腺良性病变组织(对照组)中SENP1蛋白的表达;同时采用EnVision免疫组化染色方法检测这2种组织中CerBb-2蛋白的表达。结果:SENP1蛋白在乳腺癌中的阳性率为60.78%(62/102),在乳腺良性病变中的阳性率为16.67%(5/30),两者间差异有统计学意义(P<0.01);SENP1的表达与乳腺癌分化程度、肿瘤细胞淋巴结转移情况和患者TNM分期相关(P<0.05)。乳腺癌组织中CerBb-2的阳性率为50.98%(52/102),对照组阳性率为10.00%(3/30),两者间差异有统计学意义(P<0.01)。乳腺癌组织中CerBb-2的表达与肿瘤临床分期及淋巴结转移情况相关(P<0.05)。SENP1的表达与CerBb-2的表达呈正相关。结论:SENP1和CerBb-2均对乳腺癌的增殖、生长、侵袭和转移起重要作用,有望成为肿瘤治疗的新靶点。
Objective To investigate the expression of sentrin-specific protease 1 （SENP1） and prooncogene CerBb-2 in breast carcinoma and analyze their relation with pathologic factors of breast cancer. Methods ABC immunohistochemical staining method and EnVision immunohistochemical staining method were used to detect the expressions of SENP1 protein and CerBb-2 protein, respectively, in breast cancer and benign breast lesion. Results The positive rate of SENP1 protein in breast carcinoma （62/102, 60.78%） was significantly higher than that in benign mammary lesion （5/30, 16.67%） （P〈 0.01）, and the expression of SENP1 was correlated with the degree of differentiation, lymph node metastasis and TNM stage of breast cancer （P〈0.05）. The positive rate of CerBb-2 protein in breast carcinoma （52/102, 50.98%） was also significantly higher than that in benign mammary lesions （3/30, 10.00%） （P〈0.01）, and the expression of CerBb-2 was correlated with lymph node metastasis and clinical stage of breast cancer （P〈0.05）. The expression of SENP1 was positively correlated with the expression CerBb-2. Conclusions SENP1 and CerBb-2 play an important role in the proliferation, growth, invasiveness and metastasis of breast cancer, and could potentially be the novel targets in the treatment of breast cancer.
出处
《诊断学理论与实践》
2009年第2期175-179,共5页
Journal of Diagnostics Concepts & Practice
关键词
乳腺癌
类泛素修饰
类泛素化蛋白酶
CERBB-2
免疫组织化学
Breast carcinoma
Small ubiquitin-like modifier
Sentrin-specific protease 1
CerBb-2
Immunohistochemistry
