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Aβ_(1-42)淀粉样蛋白在大肠杆菌中的可溶性表达 被引量：1

Studies on Soluble Expression of Aβ_(1-42) Amyloid Protein Gene in E. coli
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摘要 本研究中用融合标签技术通过单质粒转化和双质粒转化分别促进Aβ1-42淀粉样蛋白的可溶性表达。构建重组载体pET(yd-b42)、pET(Msb-b42)、pET(Od-b42)、pAY-sls[ydb42]、pAY-sls[tydb42],并在大肠杆菌中表达,通过SDS-PAGE验证分析。标签yd、Msb、Od、tyd都能很好的促进Aβ1-42淀粉样蛋白可溶性表达,其中yd、tyd的促溶效果最好。Aβ1-42淀粉样蛋白能在大肠杆菌中可溶性表达。为阿尔茨海默病的治疗提供进一步理论基础。 Fusion tag technology with a single plasmid transformation and two-plasmid transformation, to promote Aβ1-42 protein soluble amyloid expression. Construction of recombinant vector pET （yd-b42）, pET （Msb-b42）, pET （Od-b42）, pAY-sls [ydb42], pAY-sls[tydb42], and expressed in E. coli by SDS-PAGE to verify analysis. Tags yd, Msb, Od, tyd can promote Aβ1-42 expression of soluble amyloid, yd, tyd promote solubilization is the best. Aβ1-42 amyloid protein soluble expression in E.coli. For the treatment of Alzheimer＇s disease provide further theoretical basis.
作者 孙永红 高红亮 詹晓莹 李文娟 李雪娇
机构地区 华东师范大学生命科学学院
出处 《基因组学与应用生物学》 CAS CSCD 北大核心 2009年第2期262-264,共3页 Genomics and Applied Biology
基金 上海市科委纳米科技专项(05nm05016)资助
关键词 AΒ1-42 大肠杆菌 可溶性表达 Aβ1-42, E. coli, Soluble expression
分类号 Q78 [生物学—分子生物学]
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参考文献9

  • 1Carpentier M.,Robitaille Y.,DesCroseiilers L.,Boileau G.,and Marcinkiewicz M.,2002,Declining expression of neprilysin in Alzheimer disease vasculature:possible involvement in cerebral amyloid angiopathy,J.Neuropathol.Exp.Neurol.,61(10):849-856.
  • 2Douglas G.,1999,Cerebrospinal fluid opens a window in Alzheimer disease,Arch.Neurol.,56(6):655-656.
  • 3Hashimoto Y.C.,Niikura T.,Ito Y.,and Nishimoto I.,2000,Multiple mechanisms underlie neurotoxieity by different types of Aizheimer's disease mutation of amyloid precursor protein.,J.Biol.Chem.,275(44):34541-34551.
  • 4刘元,华茜,雷洪涛,李澎涛.老年斑β淀粉样蛋白的清除机制[J].现代生物医学进展,2008,8(1):152-154. 被引量：4
  • 5Mizushima N.,2005,A (beta) generation in autophagic vacuoles,J.Cell Biol.,171(1):15-17.
  • 6曲荟锦,王凤寰,田平芳,谭天伟.两种策略实现1,3-丙二醇关键酶基因的共表达[J].北京化工大学学报（自然科学版）,2007,34(4):421-424. 被引量：4
  • 7Weller R.O.,Massey A.,Kuo Y.M.,Mazanti I.,and Nicoll J.A.,2002,Cerebrovascular disease is a major factor in the failure of elimination of Abeta from the aging human brain:implications for therapy of Alzheimer's disease,Ann.N.Y.Acad.Sci.,977:162-168.
  • 8Wang D.S.,Dickson D.W.,and Maiter J.S.,2006,13-amyloid degradation and Alzheimer's disease,Journal of Biomedicine and Biotechnology,58:406.
  • 9张萍,张留莎,王传升.阿尔茨海默病和帕金森痴呆患者脑脊液中tau蛋白和β淀粉样蛋白水平的研究[J].中华老年心脑血管病杂志,2007,9(2):113-115. 被引量：6

二级参考文献45

  • 1宋秋杰,李林.Alzheimer病神经元凋亡的Aβ级联假说[J].中原医刊,2005,32(7):26-28. 被引量：3
  • 2张晓梅,唐雪明,诸葛斌,沈微,饶志明,方慧英,诸葛健.产1,3-丙二醇新型重组大肠杆菌的构建[J].生物工程学报,2005,21(5):743-747. 被引量：12
  • 3Brion-JP.The role of neurofibrillary tangles in Alzheimer disease[J].Acta Neurol Belg,1998,98:165-174.
  • 4Anderton-BH,Dayanandan-R,Killick-R,et al.Does dysregulation of the notch and wingless/Wnt pathways underlie the pathogenesis of Alzheimer's disease[J].Mol Med Today,2000,6:54-59.
  • 5Cotman CW.Apoptosis decision cascades and neuronal degeneration in Alzheimer's disease[J].Neurobiology of Aging,1998,19:29-32.
  • 6Kawasumi M,Hashimoto Y,Chiba T,et al.Molecular mechanisms for neuronal cell death by Alzheimer's amyloid precursor protein -relevant insults[J].Neurosignals,2002,11:236-250.
  • 7Qiao J,Qu QM,Yang JB,et al.A study on the intellectual impairment in patients with Parkinson disease[J].Chin J Clin Rehabil,2002,28:43 -44.
  • 8Selkoe DJ.Deciphering the genesis and fate of amyloid beta-protein yields novel therapies for Alzheimer disease[J].J Clin Invest,2002,110:1375-1381.
  • 9Weller RO,Massey A,Kuo YM,et al.Cerebrovascular disease is a major factor in the failure of elimination of Abeta from the aging human brain:implications for therapy of Alzheimer's disease[J].Ann NY Acad Sci,2002,977:162-168.
  • 10Carpentier M,Robitaille Y,DesCroseillers L,et al.Declining expression of neprilysin in Alzheimer disease vasculature:possible involvement in cerebral amyloid angiopathy[J].J Neuropathol Exp Neurol,2002,61:849-856.

共引文献11

同被引文献16

  • 1黎名元,郭新民,乔传令.重组大肠杆菌高密度培养研究进展[J].微生物学杂志,2006,26(6):55-58. 被引量：4
  • 2肖洁,郭刚,邹全明.提高大肠杆菌分泌表达重组蛋白的研究进展[J].微生物学杂志,2007,27(2):73-77. 被引量：9
  • 3Liao Y D, Jeng J C, Wang C F, et al. Removal of N - terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase. Protein Science, 2004, 13 (7) : 1802.
  • 4Shokri A, Sanden A M, Larsson G. Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli. Appl Microbiol Biotechnol, 2003, 60 (8) : 654.
  • 5Mergulhao F J M, Summersb D K, Monteiro G A. Recombinant protein secretion in Escherichia coli. Biotechnology Advances, 2005, 23:177.
  • 6Choi J H, Jeong K J, Kim S C, et al. Efficient secretory production of alkaline phosphatase by high cell density culture of recombinant Escherichia coli using the Bacillussp Endoxylanase signal sequence. Appl Microbiol Biotechnol, 2000, 53 (6) : 640.
  • 7Jeong K J, Lee S Y. Excretory production of humanβ2 endorphin into euhure medium by using outermembrane protein Fas a fusion partner in reeombinant Escherichia coli. App 1 Environ Microbiol, 2002, 68 (10) : 4979.
  • 8Yamabhai M, Emrat S, Sukasem S, et al. Secretion of recombinant Bacillus hydrolytic enzymes using Escherichia cob expression systems. Journal of Biotechnology, 2008, 133 : 50.
  • 9Si Jae Park, Sang Yup Lee. Efficient recovery of secretory recombinant proteins from protease negative mutant Eseherichia colistrains. Biotechnology Techniques, 1998, 12( 11 ) : 815.
  • 10Sandee D, Tungpradabkul S, Kurokawa Y, et al. Combination of Dsb coexpression and an addition of sorbitol markedly enhanced soluble expression of single chain Fv in Escherichia coli. Biotechnol Bioeng, 2005, 91 (4) :418.

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基因组学与应用生物学
2009年 第2期

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