摘要
目的研究SIGIRR对未甲基化的胞嘧啶磷酸鸟苷（CpG）诱导的人气道上皮细胞株H292细胞钟形受体9（TLR9）表达的影响。方法本实验对象包括6组，分别为H292组、空质粒转染组（eH292组）、SIGIRR转染组（SH292组），以及给予CpG刺激后的H292组（CH292组）、eH292组（CeH292组）和SH292组（CsH292组）。构建SIGIRR-EGFP融合蛋白的真核表达载体，运用脂质体转染的方法转染人气道上皮细胞株H292经CpG刺激后，Western blot检测人气道H292细胞TLR9的表达，ELISA检测人气道上皮细胞H292分泌IL-6的水平。结果CH292组、CeH292组和CsH292组细胞较H292组、eH292组、sH292组细胞TLR9蛋白表达量增加（P均〈0．01）；CSH292组细胞产生的IL-6（3．41±0．08pg／ml）少于CH292组（4．27±0．07pg／ml）及CeH292组（5．04±0．05pg／ml）（P均〈0．05）、而TLR9蛋白表达量差异无统计学意义。结论CpG可诱导Hm细胞表达TLR9蛋白，SIGIRR上调表达可抑制CpG诱导的H292细胞IL-6的产生，对TLR9表达无影响，
Objective To investigate effects of SIGIRR （single Ig IL-1R-related molecule） on TLR9 expression in human airway epithelial cells H292 induced by CpG. Methods The study was conducted in 6 groups, including H292, eH292, sH292, CH292, CeH292 and CsH292 groups. An eukaryotic expression vector was constructed for single Ig IL-1R-related molecule fused with enhanced green fluorescent protein （EGFP） gene, and transfected into H292. IL-6 level was then detected by ELISA, and TLR9 protein expression was observed by Western blot m human airway epithelial cells H292 induced by CpG. Results TLR9 protein expression in CH292, CeH292 and CsH292 groups was significantly higher than that in H292, eH292 and sH292 groups （all P〈0.01）. IL-6 level in CsH292 group （ 3.41 ＋0.08 pg/ml ） was significantly lower than that in CH292 group （ 4.27 ＋0.07 pg/ml ） and CeH292 group （ 5.04 ＋ 0.05 pg/ml ） （ both P 〈 0.05 ）, but TLR9 expression in CsH292 group was not significantly different from that in CH292 and CeH292 groups. Conclusions CPG could induce TLR9 experssion in human airway epithelial cells H292, and up-regulation of SIGIRR can inhibit CpG-induced inflammation through inhibiting the production of IL-6 but not TLR9.
出处
《老年医学与保健》
CAS
2009年第2期86-88,共3页
Geriatrics & Health Care
基金
国家自然科学基金（No.30600272）
关键词
SIGIRR
受体
细胞表面
H292细胞
Single Ig IL-1R-related molecule
Receptors, cell surface
H± cell
