摘要
建立了固相萃取-高效液相色谱法测定水样中5种硝基苯类化合物，硝基苯、2，4，6-三硝基甲苯、2，4-二硝基氯苯、邻-硝基氯苯和对-硝基氯苯的方法。对固相萃取，水样顸处理和色谱分离条件做了试验并予以优化。采用Porapak RDX固相萃取柱将样品浓缩富集后，以C18色谱柱（250mm×．6mm，5μm）为分离柱，以甲醇和水以体积比55比45的混合液为流动相，在检测波长为260nm处进行测定。方法检出限（2S／N）为0．8～1．8μg·L^-1，相对标准偏差（n=7）在1．1％～5．6％之间，加标回收率在81．5％～101．0％之间。该方法已用于地表水及生活饮用水中的硝基苯类化合物测定。
SPE-HPLC was applied to the determinations of nitrobenzenes in water, including nitrobenzene, 2,4,6-trinitrotoluene, 2, 4-dinitrochlorobenzene, 2-chloronitobenzene and 4-chloronitobenzene. The conditions of pretreatment of water sample solid phase extraction, and separation by liquid chromatography were tested and optimized. Nitrobenzenes in sample was enriched on the Porapak RDX column, and separated on C18 column （250mm×4.6mm,5μm）. A mixed solution of methanol and water （mixed in the ratio of 55 to 45） was used as mobile phase. UV-detection at 260 run was adopted in the determination. Detection limit （2S/N） of the method was found in range of 0. 8-1.8μg·L^-1. Tests for recovery and precision were performed, giving results of recovery in the range from 81.5% to 101.0% and value of RSD＇s （n=7） in range from 1.1% to 5. 6%. The proposed method has been used in determinations of nitrobenzenes in samples of surface water and drinking water.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2009年第4期425-427,430,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
