摘要
以法兰地草莓为试材,研究了不同6-BA浓度、不同碳源、不同基本培养基对法兰地草莓花药愈伤组织诱导的影响,不同组合的细胞分裂素对愈伤组织诱导分化的影响。结果表明:6-BA浓度1mg/L、麦芽糖作碳源、MS为基本培养基最适合法兰地草莓愈伤组织诱导。细胞分裂素以6-BA2mg/L+ZT2mg/L组合最适合诱导不定芽分化。试验中观察到:法兰地草莓花药直接接种于MS+6-BA1mg/L+NAA0.2mg/L+麦芽糖30g/L的培养基上,70d后可直接诱导出不定芽,其分化率高达11%。
Took Falandi Strawberry as an experimental material, we made a research on the different densities of 6-BA, different carbon sources and different basic culture mediums＇ influence on Falandi Strawberry Anther＇s wound recovery tissue inducement. We also studied different combinations of cell division＇s influence on the division of wound recovery tissue inducement. The result showed that 6-BA density 1 mg/L, malt sugar as carbon source, and MS as basic culture medium were most suitable for Falandi Strawberry Anther% s wound recovery tissue inducement. As to cell division, 6-BA 2 mg/L＋ZT 2 mg/L combination was the best way for the division of inducing adventitious buds. From the ex- periment we observed that directly inoculating Falandi Strawberry anther on the culture medium of MS＋6-BA 1 mg/L＋ NAA 0. 2 mg/L plus culture medium of malt sugar 30 g/L, 70 days later adventitious buds could be induced, and the rate of division tops 11%.
出处
《北方园艺》
CAS
北大核心
2009年第4期83-84,共2页
Northern Horticulture
关键词
法兰地草莓
花药培养
愈伤组织诱导
不定芽分化
Falandi strawberry
The culture of anther
Wound recovery tissue inducement
The division of adventitious bud
