摘要
本试验结果表明，基本培养基的愈伤组织诱导率，Ｂ５明显地高于ＭＳ、Ｎ６、Ｅ１。［ＮＯ３-］／［ＮＨ4＋］值大，有利于愈伤组织的诱导。乳糖作为碳源，其诱导愈伤组织的效果明显优于蔗糖。花药愈伤组织诱导率的高低与基因型和培养基有关。ＭＳ＋ＢＡ２．０ｍｇ·Ｌ－１＋ＩＡＡ４．０ｍｇ·Ｌ－１＋ＺＴ２．０ｍｇ·Ｌ－１适于作不定芽的分化培养基，其分化率达４０．００％。
According to the results of this study,the strawberry callus induction frequency on basic medium B,was significantly higher than that on MS,N6 or E1. The high quotient of [NO]/[NH] was helpful to induce calli.As carbon source in medium to induce calli,lactose was superior to sucrose.The anther callus induction frequency was related to genotype and medium.The optimum medium for plantlet differentiation was the MS medium supplemented with 2.0 mg.L-1 BA+2.0 mg.L-1 ZT+4.0 mg.L-1 IAA,giving a differentiation rate of 40.00%.
出处
《上海农业学报》
CSCD
1995年第3期27-30,共4页
Acta Agriculturae Shanghai
关键词
草莓
花药培养
愈伤组织
诱导
碳源
不定芽
Strawberry
Anther culture
Callus induction
Adventitious bud
Carbon source in medium
