摘要
目的建立测定芬布芬片含量的高效液相色谱(HPLC)法。方法采用Agilent C18柱(150mm×4.6mm,5μm),流动相为甲醇-乙腈-水(40∶60∶40,用磷酸调节pH至3.0),检测波长为281nm,流速1.0mL/min,柱温30℃。结果芬布芬进样量线性范围为0.076～0.918μg(r=0.9999),平均回收率为99.14%,RSD为0.59%(n=6)。结论HPLC法简便、准确,可作为芬布芬片的质量控制方法。
Objective To establish a HPLC method for the determination of Fenbufen Tablets. Methods The Agilent C18 column（150 mm × 4.6 mm, 5 μm） was used, the mobile phase was methanol- acetonitrile- water（40 ： 60：40, adjusting to pH =3.0 with phosphoric acid）, with detection at 281 nm, the flow rate of 1.0 mL/min and the column temperature at 30℃. Results The linear range of fenbufen was 0.076-0. 918 μg （r=0.999 9）, the mean recovery rate was 99. 14% RSD was 0. 59% （n=6）. Conclusion This method is convenient and accurate. It can be used for the quality control of Fenbufen Tablets.
出处
《中国药业》
CAS
2009年第8期30-30,共1页
China Pharmaceuticals
