摘要
【目的】研究谷氨酰胺(Gln)对小鼠肝脏、子宫、卵巢组织热休克蛋白70(Hsp70)表达以及对小鼠早期胚胎体外发育的影响。【方法】选取24只8w健康的雌性昆明小白鼠并随机分成4组,Ⅰ组为对照组,尾静脉注射生理盐水,Ⅱ、Ⅲ、Ⅳ组分别注射不同剂量的Gln,注射后4h处死,取肝脏、子宫和卵巢,采用RT-PCR和WesternBlot法检测HSP70mRNA和HSP70的表达;用添加不同浓度Gln的葡萄糖-CZB培养液对小鼠早期胚胎进行体外培养,观察囊胚发育率和孵出率,用WesternBlot方法检测小鼠囊胚Hsp70的表达。【结果】Gln注射组鼠组织中Hsp70表达量均高于对照组,且Hsp70表达量随着Gln注射量的增加而增加。Ⅱ组鼠肝脏、子宫和卵巢组织中Hsp70蛋白表达量比对照组分别增加了9.49%、5.49%和4.84%(P>0.05);Ⅲ组鼠各组织中Hsp70比对照组分别显著(P<0.05)增加了23.56%、21.10%和14.30%;Ⅳ组肝脏组织中HSP70比对照组显著增加了35.33%(P<0.05),子宫和卵巢比对照组分别极显著(P<0.01)增加了33.94%和33.77%;Gln为6和10mmol·L-1的胚胎培养液中小鼠囊胚孵出率分别为73.21%和76.41%,显著(P<0.05)高于对照组(61.88%)。【结论】Gln能诱导小鼠肝脏、子宫、卵巢组织和囊胚Hsp70的表达,并且Hsp70表达量随着Gln剂量的加大而增加;Gln对体外培养的小鼠早期胚胎的发育有明显促进作用。
【Objective】 The objectives of this study were to study the effect of glutamine on both the expression of Hsp70 in the tissues of mice liver, uterus, ovary and in the early embryos, and the development of mice early embryos in vitro. 【Method】 Twenty-four healthy, 8-week-old male Kunming mice were used and randomly divided into 4 groups. GroupⅠwas used as control and was injected with 0.5 ml of saline through vena caudalis. Groups Ⅱ, Ⅲ and Ⅳ were injected with different doses of Glutamine, respectively. The mice were put to death 4 h after injection and the samples of liver, ovary and uterus tissues were taken, respectively. Heat shock protein 70 expression was detected by both RT-PCR and Western blot. The early embryos were cultured in vitro in G-CZB supplemented with different amounts of Gln. Blastocyst rates and hatched blastocyst rates were counted and the heat shock protein 70 of the embryos was detected by Western blot. 【Result】 The expression of Hsp70 in the groups of mice injected with Gln were all higher than that in the control group, and the expression of Hsp70 increased with the increased amount of Gln injected. The expression of Hsp70 in liver, uterine and ovarian tissues increased by 9.49%, 5.49% and 4.84% （P〉0.05）, respectively, in group Ⅱ and increased by 23.56%, 21.10% and 14.30% （P〈0.05）, respectively, in group Ⅲ as compared with that in groupⅠ. And in group Ⅳ it increased by 35.33% （P〈0.05）, 33.94% （P〈0.01） and 33.77% （P〈0.01）, respectively. The blastocyst rate and hatched blastocyst rate were significantly higher when the embryos were cultured in the G-CZB supplemented with 6 mmol·L^-1 （73.21%） and 10 mmol·L^-1 （76.41%） of Gln, respectively, than that （61.88%） in the control group. 【Conclusion】 The expression of Hsp70 in mice liver, uterine and ovarian tissues and in mice blastocysts can be induced by Gln, and the expression of Hsp70 increased with the increased amount of Gln. Gln can promote the development of mice early embryos cultured in vitro.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第4期1428-1434,共7页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(30871893)
