摘要
研究了不同浓度盐胁迫下甜高粱幼苗的抗性酶的活性及MDA和可溶性蛋白的含量。甜高粱幼苗采用水培的方法,设3个盐梯度,1个对照。随着NaCl胁迫的加剧(当NaCl浓度大于50mmol/L时)甜高粱幼苗膜脂过氧化程度不断加大,膜系统受到破坏;膜保护酶(POD,SOD,CAT,APX)活性降低,200mmol/L NaCl处理的酶活下降最大,100mmol/L NaCl处理次之,使体内酶促和非酶促防御系统均遭到破坏,活性氧含量增加对幼苗产生毒害。其中,100mmol/L NaCl处理后膜脂过氧化程度较轻,膜系统受害程度也轻于200mmol/L NaCl处理。轻度盐胁迫(当NaCl浓度是50mmol/L)时,由于抗性酶的作用,活性氧对膜系统没有产生破坏作用。随着盐浓度的增加,抗性酶的活性降低,活性氧的含量不断增加,膜系统受破坏程度也不断加大。
The resistance enzymes activity, MDA and soluble protein contents effects of salt-stressed on sweet sorghum seedlings was discussed in the paper. Sweet sorghum seedlings different degree were cultured in the water, we set up three salt degrees, and one CK. Above 50 mmol/L NaCl resulted in membrane lipid per-oxidation, and membrane system is destroys, the action of POD, SOD, CAT and APX declined. Enzyme action of 200 mmol/L NaCl treatments has the largest descendence.100 mmol/L NaCl treatments are less than 200 mmol/L NaCl treatments. In the light salt-stressed （when NaCl concentration was 50 mmol/L）, active oxygen did not destroy the membrane system, as a result of resistance enzymes. By the increasing of salt concentration, resistance enzymes activity declined, active oxygen content arose, and the destroy degree of membrane system increased too.
出处
《中国农学通报》
CSCD
北大核心
2009年第6期136-139,共4页
Chinese Agricultural Science Bulletin
关键词
NACL胁迫
抗性酶
甜高粱
NaCl-stress, resistance enzymes, sweet sorghum
