摘要
目的为临床应用自体肝细胞脾内移植治疗急性肝衰竭探索新路。方法用Ｗｉｓｔａｒ大白鼠制备急性外科型肝衰竭模型，采用肝中叶门静脉分枝插管，用Ⅰ型胶原酶灌注制备游离肝细胞，行自体肝细胞脾内移植。结果病理组织学观察移植组１０周后大鼠脾内有弥散状、团状或单排列状肝细胞存在；同位素９９ｍＴｃ－ＨＩＤＡ测定证实其肝功能接近正常；对照组术后２４ｈ死亡３０％，４８ｈ累计死亡５５％，移植组分别为２０％和３０％。结论实验提示脾内移植肝细胞可以存活且有一定代谢功能。
Objective To study the new way of the intrasplenic autotransplantation of liver cell for the treatment of acute hepaticfailure for the clinical application.Method Surgical pattern induced acute hepatic failure was made in the Wistar rats. The tube was insertinto the pylic branch in the liver middle lobulation.Free liver cell was gained by perfusion I type collagenase.The intrasplenicautotransplantation of liver cell was injected.Results Pathological findings there were dispersion, groups and single arrangement liver cell in the spleen of rats in the transplantation group after 10 weeks;Liver cell function was normal whick was determined by 99mTcHIDA isotope scintiscanning; Mortality was 30% in the comtrol group afterpostoperative 24h. Accumulation mortality was 55% during 48h.In group of transplantation was 20% and 30% respectively.Conclusion There wasmetabolism in the liver cell transplanted into the spleen. The survivalrate was significantly elevated on the acute liver failure in rats.
出处
《潍坊医学院学报》
1998年第1期30-32,共3页
Acta Academiae Medicinae Weifang
关键词
自体肝细胞
移植
肝衰竭
治疗
selfliver cell
grafting
hepatic failure
