摘要
采用Alcalase蛋白酶水解杏仁蛋白,以水解度(DH)及水解产物对血管紧张素转化酶(ACE)的抑制率为指标进行酶解工艺优化。结果表明,较大活性的ACE抑制肽的最佳水解条件为:pH值7.0,温度50℃,酶底比4%,底物质量分数为2%。该条件下经60min水解,其水解度为12.23%,得到ACE抑制肽的IC50值为0.85mg/mL。
Apricot （Prunus armeniaca L.） kernel protein was hydrolyzed with Alcalase protease. In order to optimize the hydrolysis process, the degree of hydrolysis （DH） and the angiotensin I - converting enzyme （ACE） inhibitory activity of the hydrolysates were investigated. The results indicated that, the ACE inhibitory peptides with high inhibitory activities was prepared, the optimal conditions were： pH 7.0, temperature 50 ℃ , [E]/[S]4%, substrate concentration 2%. Under such conditions, after 60 rain hydrolysis, the DH was 12.23%, and the IC50 of the ACE inhibitory peptides was 0.85 mg/mL.
出处
《农产品加工（下）》
2009年第3期149-152,共4页
Farm Products Processing
