摘要
目的研究ras癌基因及p53抑癌基因在在胰腺癌表达的临床意义．方法胰腺石蜡包埋标本55例，包括胰腺癌32例，癌切缘未受浸润的胰腺组织12例，急慢性胰腺炎7例，正常胰腺4例，标本均经病理学证实，用免疫组织化学ABC法对上述标本的ras癌基因及p53抑癌基因表达进行检测．结果在32例胰腺癌中，ras和p53基因的阳性表达率分别为71．9％和28．1％，高于胰腺炎和癌切缘组织（P＜0．05）ras，p53基因表达与患者性别、年龄、肿瘤所在部位及大小和初发症状无关（P＞0．05），p53基因表达与临床分期和病理分级相关（P＜0．05），p53基因表达阳性者其临床分期较晚期，细胞分化较差，而ras基因表达仅与病理分级有关（P＜0．05）．P53基因表达与癌肿可切除率相关（P＜0．05），凡癌肿能切除者多为p53基因表达阴性者.p53基因表达亦与淋巴结转移有关（P＜0．05），其表达阳性者多已有淋巴结转移．ras与p53基因表达之间存在正相关关系．结论ras与p53基因表达可以反映胰腺癌的生物学行为，对胰腺癌的诊断和治疗有一定指导意义．
AIM To study the clinical significance of ras oncogene and p53 antioncopene expression in pancreatic carcinomas.WETHODS The ras oncogene and p53 antioncogene expressions of 55 pancreatic paraffin-embedded.specimens, included 32 carcinomas.were studided by immunohistochemistry ABC method.12 specimens taken from the normal pancreatic tissue nearby the tumor transection margin, 7 specimens of pancreatitis and 4 specimens of normal pancreas were compared as contrls.RESULTS The positive expression rate of ras oncogene and p53 antioncogene was 71.9% an 28.1% in 32 cases of pancreatic carcinomas and it is higher than that of the pancreatitis and normal pancreatic tissue nearby the tumor transection margin (P<0.05). The ras and p53 gene expression wasn't significantly related to sex,age,site, size and incipient symptoms (P> 0.05). The p53antioncogene expression was related to tumor staging and grading (P<0.05). The tumor mass with negative p53 gene expression usually had a higher resectability (P<0.05). and its positive expression usually associates with lymph node metastasis (P<0.05), and worse prognosis.lt also provided a important guide to choose the methods of treatment.CONCLUSION The ras and p53 gene expression could be used to evaluate the pancreatic carcerous biological behaviour, and it might direct the diagnosis and treatment methods to pancreatic carcinomas.
出处
《世界华人消化杂志》
CAS
1998年第S2期237-239,共3页
World Chinese Journal of Digestology
