摘要
目的观察肝细胞癌（HCC）癌分组织与单纯肝硬变二者在肝细胞凋亡的区别，以探讨癌前病变的特性．方法HCC癌旁标本及单纯肝硬变标本分别为24及对例（A及B组），全部作原位末端标记（ISEL），HBsAg免疫组化及HE观察分析ISEL阳性强度分4级，作A、B组比较，卡方统计．结果A组的肝细胞ISEL阳性率及阳性强度均显著高于B组（P＜0．01）．阳性细胞核深棕色，散在分布，以汇管区及纤维隔周边部为多．A组绝大多数属静息性门脉性肝硬变；而B组尚有慢活肝及侵重肝肝硬变炎症B组较A组明显（P＜0．05）．HBsAg阳性率A组89．4％，B组93．7％ISEL阳性与HBsAg、肝细胞活跃增生和肝细胞非典型增生无相关性此外，发现A组中有1例“凋亡性肝硬变”，其ISEL及HE均证明50％的肝细胞有凋亡结论HCC癌旁肝硬变的凋亡发生率明显高于单纯肝硬变者，认为肝细胞凋亡是HCC癌前病变的一种特性，与癌的发生有关．
AIM To study the differences between hepatocyte apoptosis in par-cancerous tissue of hepatocellular carcinoma (HCC) and that in ncocancer liver cirrhosis, in order to probe the properties of precancerous lesion of HCC.METHODS Para-HCC specimens (24 cases, Group A)and noncancer cirrhosis (33 cases, Group B) were all tested by in situ terminal end labeling (ISEL), HBsAg immunohistochemistry and HE analysis. ISEL (+ )intensity was divided into 4 grades. The results of the 2 groups were compared.RESULTS The positivity rates and positive intensity of ISEL in Group A were significantly higher than that in Group B (p<0.01). The positive cell nuclei tended to scatter just near the-septa and portal tracts. The majority of Group A were of static portal cirrhosis, while Group B also included cirrhosis of chronic active hepatitis and chronic severe hepatitis. lnflammatory cell infiltration was more evident in Group B than Group A (P<0.05 ).The HBsAg(+) rates of both Group A and Group B were very high. The ISEL positivity and HBSA, proliferation and dysplasia of hepatocyte had no correlation. There was 1 case of 'apoptotic cirrhosis' in Group A. About half of the hepetocytes in this case underwent apoptosis identified by both ISEL and HE.CONCLUSION Apoptosis is much more in para-HCC than in non-cancer cirrhosis. Hepatocyte apopotosis is one of the properties of precanceroos lesioo of HCC and be related to carcinogenesis.
出处
《世界华人消化杂志》
CAS
1998年第S2期160-162,共3页
World Chinese Journal of Digestology
关键词
肝肿瘤
癌前病变
细胞凋亡
癌.肝细胞
肝硬化
liver neoplasms
carcinoma,hepatocellular
precancerous lesions
apoptosis
liver cirrhosis
