摘要
为了研究乙醇醛聚合牛血红蛋白 ( poly Hb)对 Hb空间结构的影响 ,用傅里叶变换红外光谱仪分析比较了天然牛 Hb和聚合牛 Hb的二级空间结构。结果表明天然牛 Hb和聚合牛 Hb的 α螺旋无明显区别 ,但聚合程度高 ,将改变 Hb的基本构象 ,从而导致生理功能的变化 。
To investigate the effect of polymerized bovine hemoglobin with glycolaldehyde on conformation of bovine Hb. The secondary structure of polyHb was analysed with Fourier transform infrared spectrometer. The results showed that carrying out polymerization too far, the α helix and random coli would be changed, but these changes occurred mainly at the inner of polyHb whose surface conformation might be less affected and would be the same with nature bovine hemoglobin. This study suggests that we should be control polymerized bovine Hb with high molecular weight and remove them from the products of polymerization.
出处
《生物技术通讯》
CAS
1999年第2期134-137,共4页
Letters in Biotechnology
关键词
聚合牛血红蛋白
二级结构
红外光谱仪
polymerized bovine Hb
secondary structure
Fourier transform infrared spectrometer
