摘要
目的：对室内选育的淡色库蚊抗溴氰菊酯品系（抗性系数５１８）进行遗传分析。方法：采用ＬＣ－Ｐ值分析法研究淡色库蚊溴氰菊酯抗性的形式遗传。采用抑制剂增效试验探讨抗性机制。结果：正交和反交Ｆ１代的抗性系数分别为１５４．８和１５９．２。Ｆ１代的ＬＣ－Ｐ线靠近抗性亲本一方，Ｄ＝０．６２５；ＢＣ代在死亡率５０％处、Ｆ２代在死亡率２５％和７５％处未出现明显平坡，ＢＣ和Ｆ２的实测曲线和理论曲线均存在较大差异，Ｐ均＜０．０１；ＰＢ、ＤＥＦ和ＴＰＰ对抗性品系的增效比分别为２７．１３、１．２２和１．０５。结论：淡色库蚊对溴氰菊酯的抗性为多基因遗传，其主要基因为不完全显性，多功能氧化酶是抗性的主要因子。
AIM: To analyse the genetic character of deltamethrin resistant laboratory strain (resistant factor being 518) of Cx.pipiens pallens. METHODS: Genetic studies were made of deltamethrin susceptible and resistant strains of Cx.pipiens pallens with LC P curve contructed from the response of fourth instar larvae to deltamethrin treatment, and the resistance mechanisms were analysed with synergetic tests in PB, DEF and TPP. RESULTS: The resistant factors of F1 generation from cross and reciprocal cross are respectively 154 8 and 159 2; the LC P curve of F1 generation gets near to that of resistant parents, D=0 625; there exist no obvious platform in 50% mortality of BC generation and 25% and 75% mortality of F2 generation, and there are larger differences between the determining curve and theoretical curve in both BC and F2 generations, P<0 01; the synergetic ratioes of PB, DEF and TPP are 27 13, 1 22 and 1 05. CONCLUSION: Deltamethrin resistance of Cx.pipiens pallens is controlled by two or more autosomal genes, and the main gene involved is incompletely dominant; multi functional oxidase is mainly responsible for the resistance.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1998年第1期21-24,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
淡色库蚊
溴氰菊酯
抗性
多基因遗传
Cx.pipiens pallens, deltamethrin resistance, multiple gene inheritance, incomplete dominance, multi functional oxidase
