摘要
目的观察天花粉蛋白经由ＴＣＲ／ＣＤ３介导的信号传递途径抑制Ｔ细胞活化增殖的效应。方法用各种不同的剌激信号来激活外周血淋巴细胞，然后观察天花粉蛋白对其增殖抑制的影响。接着测定天花粉蛋白对ＣＤ３单抗剌激后Ｔ淋巴细胞内一些信号传导物质变化的影响。结果天花粉蛋白抑制经由ＴＣＲ／ＣＤ３信号传导途径诱发的淋巴细胞增殖。进而发现细胞内游离钙离子的升高，蛋白激酶Ｃ的转位和细胞内蛋白酪氨酸磷酸化受天花粉蛋白的抑制。结论天花粉蛋白可通过ＴＣＲ／ＣＤ３的信号传导途径抑制Ｔ细胞增殖。
Trichosanthin can inhibit the lymphoproliferation in vitro via immunosuppression. In this paper,PBMC were stimulated with different stimuli and the mechanism of inhibition was studied.Our results showed that the inhibition of lymphoproliferation by Trichosanthin was resulted from interfering with the signaling pathway directed by TCR/CD3 complex.Moreover, there were alternations of signaling events in antiCD3 stimulated PBMC after Trichosanthin treatment,which included a raising of cytosolic Ca2+,the suppression of PKC translocation and a decreased phosphorylation of protein tyrosine kinase.It suggests that Trichosanthin can suppress T cell activation by means of interfering with signal transduction.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1998年第2期111-114,共4页
Chinese Journal of Microbiology and Immunology
关键词
天花粉蛋白
免疫抑制
信号传导
T细胞活化
richosanthin Immunosuppression Signal transduction Protein kinase C
