摘要
目的阐明３７．４ＧＨｚ毫米波对胚胎产生损伤效应的阈值及导致成年子鼠学习记忆功能降低的机理。方法用频率３７．４ＧＨｚ、功率密度１、３、５、８ｍＷ／ｃｍ２的毫米波，在小鼠怀孕６～１５ｄ时进行２ｈ／ｄ辐射，用Ｙ型电迷宫对成年子鼠进行学习记忆功能测试，分别用ＲＢＡ、ＨＰＬＣＥＣＤ、ＲＩＡ法对足孕胚胎和成年子鼠脑ＭＲ、单胺递质和ＡＶＰ进行测定。结果５、８ｍＷ／ｃｍ２的毫米波导致成年子鼠学习记忆功能明显降低、成年子鼠垂体中ＡＶＰ含量明显降低，成年子鼠海马区Ｂｍａｘ值明显升高、成年子鼠脑中多巴胺（ＤＡ）含量及足孕胚胎脑中ＤＡ含量明显降低，而１、３ｍＷ／ｃｍ２毫米波未导致以上指标的明显变化，以上指标均与功率密度及学习记忆成绩呈明显相关。结论３７．４ＧＨｚ毫米波辐射孕鼠导致胚胎损伤效应的阈值在３～５ｍＷ／ｃｍ２之间，其导致成年子鼠学习记忆功能降低与使其脑中ＡＶＰ、ＤＡ含量降低及使海马区胆碱能神经活性降低密切相关。
Objective To determine the threshold of fetus injury induced by millimeter wave irradiation with a frequency of 37.4 GHz,and to clarify the mechanism of lowering learning capacity and memory of the adult offspring. Methods Twomonth old mice were separately irradiated by millimeter wave with a frequency of 37.4 GHz at power densities of 1,3,5, 8 mW/cm2 for 2h daily from the 6th to 15th day of gestation. The test of learning capacity and memory of adult offspring was employed on Ytype electric maze. The quantity of Mcholinergic receptor(MR) and its affinity to ligand, the contents of monoamine transmitter and argininevasopressin(AVP) in the brain of the fetuses at term, adult offspring were measured by using radioligand birding assay(RBA), high proficient liquid chromatographyelectrochemical determination (HPLCECD) and radioimmunoassay(RIA) separatively. Results Millimeter wave irradiation at power densities of 5,8 mW/cm2 lowered learning capacity and memory of adult offspring. 8mW/cm2 significantly reduced the content of AVP in pituitary gland of adult offspring,meanwhile increased quantity of MR in hippocampus of adult offspring.It obviously reduced the content of DA in brain of adult offspring and the fetuses at term. But, millimeter wave irradiation at power densities of 1, 3 mW/cm2 caused no significant changes of aforesaid parameters. There is a significant correlation among the aforesaid parameters, power densities and learning capacity and memory results. Conclusion The threshold of fetus injury induced by millimeter wave irradiation to pregnant mice with a frequency of 37.4GHz is between 3 mW/cm2 and 5 mW/cm2. The mechanism that millimeter wave irradiation lowered learning capacity and memory of the adult offspring is closely related to a decrease of the content of AVP,DA in the brain,as well as a reduced function of cholinergic nerves in the hippocampus.
出处
《中华物理医学杂志》
CAS
CSCD
1998年第1期1-4,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金
关键词
毫米波
副作用
胎儿
学习
millimeter wave*/adv eff
fetus/rad eff
pregnancy/rad eff
learning/rad eff
mice
