摘要
目的观察中药芪红合剂在肾间质纤维化过程中对致纤维化因子结缔组织生长因子(CTGF)表达的影响,探讨其可能的肾保护作用机制。方法采用单侧输尿管结扎(UUO)致肾间质纤维化的动物模型,40只雄性Wistar大鼠随机分为4组:假手术组(A组)、手术模型组(B组)、芪红合剂组(C组)、依那普利组(D组)。术后14d处死各组大鼠,收集血清测定血肌酐(Scr)、尿素氮(Bun)水平,取结扎侧肾组织分别用HE、Masson染色,采用免疫组化方法检测肾组织中结缔组织生长因子(CTGF)的表达,并用计算机图像分析系统进行分析。结果模型组大鼠肾组织中CTGF的表达较假手术组显著升高,两治疗组较模型组明显降低。模型组大鼠血肌酐、尿素氮水平较假手术组明显增高,两治疗组较模型组显著下降。结论中药芪红合剂可降低大鼠血尿素氮、血肌酐水平,并可通过抑制CTGF的表达,而起到减轻肾间质纤维化病变程度的作用,推测其抑制CTGF表达上调的作用可能是其抗肾小管间质纤维化的作用机制之一。
Objective To observe the effect of Astragalus and Safflower mixture on the expression of CTGF in rats with Unilatreal Ureteral Obstruction. To investigate the theraputic effects and its mechanisms of Astragalus and Safflower mixture.Methods Rat renal interstitial fibrosis model was produced by unilateral ureteral obstruction,40 Wistor male rats were randomly divided into four groups ： sham group（A groap）,UUO group（B group）,A＆S group（C group） and ACEI group（D group）.Sera and kidney tissues were collected from each group on the forteenth day.Bun and Scr were measured. It was carried out to measure the level of tubulointerstitial damage by Masson staining. The expression of CTGF in kidneys was analyzed by inununohistochemical method. Results The expression of CTGF and the renal interstitial fibrotic area were bighter significantly than that in sham group. Compared with UUO group,those of the treatment groups decreased markedly. The level of BUN and Scr in UUO group were significantly highter than that in sham group.Compared with UUO group,the level of BUN and Scr in treatment groups were significantly lower. Conclusion Astragalus and Safflower mixture can downregulate the level of BUN and Scr in UUO animal model rats,Astragalus and Safflower mixture can suppress the upregulation of CTGF expression in kidneys of UUO animal model rats and decrease their interstitial fibrotic area.It is inferred that it may he one of the mechanisms of Astragalus and Safflower mixture suppressing renal tubular damage and interstitial fibrosis.
出处
《中外医疗》
2009年第4期10-12,共3页
China & Foreign Medical Treatment
基金
山西省教育厅基金资助项目。
关键词
单侧输尿管结扎
肾间质纤维化
芪红合剂
结缔组织生长因子
Unilateral Ureteral Obstruction
Renal tubulointerstitial fibrosis
Astragalus and Safflower mixture
Connective tissue growth factor
