摘要
采用荧光显微技术和电镜扫描技术 ,对 33个温湿度组合条件下球孢白僵菌孢子在马尾松毛虫活体上的萌发、侵染进程和致病效果进行了观察与测定 ,分析了三者之间的关系 .揭示了孢子萌发速率与环境温度的关系 :V =(T - 4.2 5) /2 7.2 .其中 ,孢子萌发的起点温度为 4.2 5℃ ,有效积温为 2 7.2日度 .湿度是孢子萌发和侵染致病的主要制约因子 ,且温度和湿度具有互补与综合作用 .温度达 1 0℃以上 ,相对湿度达 95% ,侵染致病效应更为显著 .
By means of fluorescence microscopic and electron scanning microscopic techniques, the conidium germination, infection process and pathogenic effect of Beauveria bassiana on the larvae of Dendrolimus punctatus were observed and tested under conditions of 33 combinations of temperature and humidity. The results show that the relationship between germination velocity(V) of the conidia and temperature(T) was determined as V=(T-4.25)/27.2, where 4.25℃ was the initial temperature, and 27.2 was the effective accumulated temperature in a day for conidium germination. Humidity was the main restrictive factor for the germination and infection of conidia, and temperature and humidity had compensatory and comprehensive effects. Pathogenic effect could occur remarkably under the condition of above 10℃ and 95% relative humidity.
出处
《应用生态学报》
CAS
CSCD
1998年第2期195-200,共6页
Chinese Journal of Applied Ecology
基金
国家"八五"科技攻关项目!( 85-1 8-0 3 -0 2 )
关键词
球孢白僵菌
马尾松毛虫
侵染效应
Beauveria bassiana, Dendrolimus punctatus, Fluorescence microscope techniques, Infection effect.
