摘要
采用１４种限制性内切酶，对号鸟形目８种鸟类（Ｔｙｔｏａｌｂａ、Ｔｃａｐｅｎｓｉｓ、Ｏｔｕｓｂａｋｋａｍｏｅｎａ、Ｏｓｃｏｐｓ、Ａｓｉｏｏｔｕｓ、Ａｆｌａｍｍｅｕｓ、Ｓｔｒｉｘａｌｕｃｏ、Ｇｌａｕｃｉｄｉｕｍｃｕｃｕｌｏｉｄｅｓ）线粒体ＤＮＡ进行限制性片段长度多态分析，结果表明：不同种间存在基因组长度多态性，短耳号鸟为２３３５ｋｂ，长耳号鸟为１９７８ｋｂ，斑头鸺留鸟为１８６２ｋｂ，红角号鸟为１７６５ｋｂ。号鸟形目种间具有较高的遗传变异，其中仓号鸟和草号鸟的平均遗传距离为１０％，长耳号鸟和短耳号鸟平均为１０８％，红角号鸟和领角号鸟平均为１３１％，灰林号鸟和斑头鸺留鸟为１２３％，其中红角号鸟与斑头鸺留鸟的平均遗传距离最大为１７５％。号鸟形目鸟类线粒体ＤＮＡ的进化速率为每百万年变化２０％～２２％，提示号鸟形目两个科间在距今２８００～３０００万年前分歧开来，同时，鸱号鸟科各种间的分歧时间在距今２０００～２５００万年前，即处于中新世中期。
Fourteen restriction endonucleases, ApaⅠ,BamHⅠ,BglⅡ,HindⅢ,HpaⅠ,KpnⅠ,PstⅠ,PvuⅡ,SalⅠ,ScaⅠ,Xba Ⅰ and XhoⅠ were used to analyse the mitochondrial DNA(mtDNA) of eight species of Strigiformes: Tyto alba,T.capensis,Otus bakkamoena,O.Scopa,Asio otus,A.flammeus,Strix aluco and Glaucidiun cuculoides . The genome size of mtDNA in Strigiformes ranged from 16 78 kb to 23 35 kb.Large size differences may be resulted from duplication or insertion of sequence fragments. High genetic diversity among species in Strigiformes was observed.From the genetic distance matrix, the average percentage divergence between A.otus and A.flammeus,O.scopa and O.bakkamoena, S.aluco and G.cuculoides was 10 8%,13 1%,12 3% respectively. The genetic distance between T.capensis and T.alba was the smallest(1 0%) , that between O.scopa and G.cuculoides was the largest(17 5%). Hypothetical mtDNA phylogenetic of Strigiformes were shown. They coincided with the result of morphological classification.According to the rate of evolution of mtDNA in Strigiformes 2 0%～2 2% per million years,the time of divergence between Tytonidae and Strigidaes was 28～30 million years ago.
出处
《动物学报》
SCIE
CAS
CSCD
1998年第1期94-101,共8页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金
