摘要
[目的]初步研究甘露聚糖酶基因3′端对甘露聚糖酶功能的重要性。[方法]以甘露聚糖酶的二级结构为基础,用PCR扩增的方法获得3′端特异性缺失的甘露聚糖酶DNA片段,连接到载体上,表达并鉴定酶的活性。[结果]删除甘露聚糖酶基因3′端246 bp后甘露聚糖酶依然具有酶活性。[结论]甘露聚糖酶基因3′端的部分序列不是酶具有功能所必需的。
[ Objective]The paper aimed to study the importance of 3＇ sequence for the function of β-mannanase. [ Method]β-mannanase DNA segement lack of 3＇ sequence was obtained by PCR amplification according to its secondary structure. The DNA was linked to vector for expressing and identifying the activity of β-mannanase. [ Result ] After 246 bp was shortened from the 3＇sequence, the activity of β-mannanase was still available. [ Conclusion] Part of the 3＇ sequence was not inevitable for the function of β-mannanase.
出处
《安徽农业科学》
CAS
北大核心
2009年第8期3496-3497,3579,共3页
Journal of Anhui Agricultural Sciences
基金
国家"863"计划课题(2006AA02Z249)
关键词
甘露聚糖酶
序列
删除
β-mannanase
Sequence
Deleting
