摘要
目的为了从分子水平探讨孕妇感染戊型肝炎病毒（ＨＥＶ）后胎儿宫内感染和死亡原因。方法对１７例患重症戊型肝炎的孕妇和娩出的１８例新生儿，以酶联免疫吸附法测母血和脐带血抗－ＨＥＶＩｇＭ／ＩｇＧ，同时查新生儿肝组织病理改变，免疫组化法查肝内戊型肝炎病毒抗原（ＨＥＶＡｇ），用地戈辛（ＤＩＧ）探针标记ＨＥＶｃＤＮＡ２３ｑｂｐ片段进行原位杂交查肝内ＨＥＶＲＮＡ。结果母血抗－ＨＥＶ、ＩｇＭ／ＩｇＧ均阳性，脐带血仅抗－ＨＥＶＩｇＧ阳性，肝组织以变性、炎变为主，新生儿肝内ＨＥＶＡｇ阳性率７７％（１０／１３），ＨＥＶ－ＲＮＡ阳性率６２％（８／１３）。
Objective To clarify causes for intrauterine infection of hepatitis E virus (HEV) and deaths of fetuses and neonates. Methods Umbilical cord blood of 18 neonates of 17 pregnant women with severe hepatitis E and their mothers′ blood were examined for anti HEV IgM/IgG by using ELISA, were studial with metbod liver biopsy specimens of the neonates, HEV antigens (HEV Ag) in the biopsy specimens, were detected by using immunohistochemical method and HEA RNA was detected by using in situ hybridization with DIG probe labelled with HEVcDNA .Results The pregnant women were positive for both anti HEV IgM/IgG in venous blood and anti HEV IgG was positive in the cord blood specimens. The histopathological examinations showed degenerations and inflammatory cells infiltration The positive rate of immunohistochemistry for HEVAg was 77% (10/13) and positive rate of in situ hybridization for HEV RNA was 62% (8/13). Conclusion These results indicated that of intrauterine infection with HEV does exist.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1998年第2期107-109,共3页
Chinese Journal of Pediatrics
基金
新疆自治区科学技术委员会资助
关键词
肝炎
疾病传播
垂直
酶联免疫吸附
测定
死胎
Hepatitise Disease transmission, vertical Enzyme linked immuno sorbent assay Fetal death
