摘要
用4种化学诱抗剂壳聚糖、草酸、硫酸亚铁和硅酸钠处理金银花,通过计算酶活性发展曲线下面积AUEAPC,分析了叶片防御酶系3种主要酶的活性变化情况。结果表明:4种诱抗剂处理中,壳聚糖处理的苯丙氨酸解氨酶(PAL)活性最高,其次是草酸处理,两者差异极显著,并且极显著高于硅酸钠、硫酸亚铁处理和对照,而后3种处理间差异不显著;硫酸亚铁处理后,过氧化物酶(POD)的活性最高,极显著高于壳聚糖、草酸和硅酸钠处理,并且这4种诱抗剂处理POD的活性均极显著高于对照;但只有壳聚糖处理,多酚氧化酶(PPO)的活性极显著高于对照,其他3种诱抗剂处理的PPO活性极显著低于对照。壳聚糖、草酸、硫酸亚铁和硅酸钠处理后,3种酶的综合活性分别比对照提高71.1%、31.1%、11.7%和2.5%,与前期对金银花白粉病诱抗效果研究结论相一致。本研究初步从防御酶学角度分析了4种化学诱抗剂在金银花上的诱导抗病机制。
The activities of three main kinds of defense-related enzymes in honeysuckle leaf were tested by the area under enzyme activity progress curve （AUEAPC） method after induction treatment using four chemical inducers chitosan, oxalic acid, ferrous sulfate （FeSO4） and sodium silicate. The results showed that the activities of phenylalanine aminolyase （PAL） was the highest followed by the oxalic acid treatment, which were significantly different from and more than the sodium silicate, ferrous sulfate treatments and the control, but the latter three treatments were not significantly different from each other. The activity of peroxidase （POD） in the ferrous sulfate treatment was significantly stronger than that of chitosan, oxalic acid and sodium silicate treatments, which were all remarkably higher than the control. But only the activity of polyphenol oxidase （PPO） increased significantly in the chitosan treatment. The others were significantly less than the control. The integrated activities of the three enzymes after induction treatments using the four inducers chitosan, oxalic acid, ferrous sulfate and sodium silicate were higher than those of the control by 71.1%, 31.1%, 11.7% and 2. 5 %, respectively, which was consistent with the earlier report about the effects of these inducers on the resistance to powdery mildew in honeysuckle. The mechanisms of induced resistance of these four chemical inducers in honeysuckle were analyzed on the basis of the activities of the three defense-related enzymes.
出处
《植物保护》
CAS
CSCD
北大核心
2009年第2期75-77,共3页
Plant Protection
基金
河南省科技攻关项目(0623033700)
关键词
金银花
化学诱抗剂
防御酶系
honeysuckle
chemical inducer
defense enzymes
