摘要
建立一种应用异硫氰酸苯酯将，L-瓜氨酸和L-鸟氨酸柱前衍生后进行定量分析的高效液相色谱法．实验结果表明，瓜氨酸在0．2～2．0mg／mL范围内线性回归显著（R^2=0．9998），平均回收率99．87％；鸟氨酸在0．2-2．0mg／mL范围内线性回归显著（R^2=0．9997），平均回收率100．92％．应用该方法对发酵液中L-瓜氨酸和L-鸟氨酸含量进行测定取得了满意的结果，能有效指导谷氨酸棒杆菌L-瓜氨酸的发酵生产．
A method for reversed-phase high performance liquid chromatography （HPLC） with phenylisothio cyanate precolumn derivation was developed for the determination of L-citrulline and L-omithine. The calibration curves of L-citrulline and L-omithine were linear over the range of 0. 2 - 2. 0 mg/mL, and the recovey rates were 99. 87% and 100.92% （n =6）, respectively. It can be used as one of the direct and reliable means for quantitative determination of L-citrulline and L-ornithine. Thus, it plays an instructive role in the production of L-citrulline by corynebacterium glutamicum.
出处
《南京工业大学学报（自然科学版）》
CAS
北大核心
2009年第2期77-80,共4页
Journal of Nanjing Tech University(Natural Science Edition)
基金
江苏省高校研究生科技创新计划支持项目
国家重点基础研究发展计划(2009CB724706)
