摘要
以低氧性敏感的网纹甜瓜(Cucumis melo var. raticulalus)品种‘西域一号’(Xiyu 1)为材料,研究了钙对低氧胁迫下幼苗生长、游离态、结合态和束缚态的腐胺(Put)、亚精胺(Spd)、精胺(Spm)含量、二胺氧化酶(DAO)和多胺氧化酶(PAO)活性、H2O2 含量的影响。结果表明:与通气对照相比,低氧胁迫处理幼苗鲜重和干重显著降低,根、叶中3种形态的Put、Spd、Spm含量显著增加,DAO和PAO活性、H2O2 含量显著提高;营养液加钙处理不但缓解了低氧胁迫对幼苗生长的抑制作用,而且幼苗游离态、结合态和束缚态Put、Spd、Spm含量显著高于单纯低氧胁迫处理,DAO和PAO活性、H2O2 含量显著低于低氧胁迫处理;低氧胁迫下缺钙处理加重了低氧胁迫对网纹甜瓜幼苗的伤害。表明在低氧胁迫下,钙参与了网纹甜瓜幼苗多胺的代谢过程,对增强幼苗耐低氧性起着重要的作用。
Calcium has been found to be a second-messenger in plants involved in the perception and regulation of responses and adaptations of plants to the environment. During this period, polyamines (PAs) accumulation, the activities of diamine oxidase (DAO) and polyamine oxidase (PAO) play an important role in increasing stress-resistance of plants. However, little is known about the effect of Ca 2+ on polyamines metabolism of seedlings under hypoxia stress. In this experiment, muskmelon cultivar `Xiyu 1' (Cucumis melo var. raticulalus) seedlings with less hypoxia-resistance under hydroponic cultivation were used to study the relationship between Ca 2+ and PAs metabolism under hypoxia stress. When seedlings grew to 3 euphylla, the seedlings were transferred from quartz sand to Hoagland nutrient solutions for preculture. After 2 days, the seedlings were divided into four sets. The first and second sets of seedlings were all cultured in normal Hoagland nutrient solutions containing 4 mmol·L -1 Ca 2+. The third set of seedlings was cultured in a calcium sufficient Hoagland nutrient solution which contained 10 mmol·L -1 Ca 2+, and the fourth set of seedlings was cultured in a calcium deficient Hoagland nutrient solution with no Ca 2+ (0 mmol·L -1 Ca 2+). The first set of seedlings was exposed to normal dissolved oxygen (DO) level by using vigorous aeration (30 min·h -1) to keep DO level at 8-8.5 mg·L -1, and seedlings in the other treatments were exposed to hypoxia stress by using DO analyzer (QUANTUM-25) to maintain DO level at (2±0.2) mg·L -1. Hence, the four treatments were control, hypoxia stress, Ca 2+ sufficient-hypoxia stress treatment, and Ca 2+ deficient-hypoxia stress treatment. After 4 d of hypoxia stress, the growth of seedlings, PAs content, DAO and PAO activities, and H_2O_2 content were all assayed. The results showed that the fresh weight and dry weight of seedlings declined significantly after 4 d of hypoxia stress, the content of three polyamines (Put, Spd, Spm) of different forms (free, conjugated, bound), DAO and PAO activities, and H_2O_2 content in roots and leaves of seedlings all increased significantly with hypoxia stress treatment compared to the control. In the calcium sufficient-hypoxia stress treatments, seedling growth under hypoxia stress was not inhibited, PAs (Put, Spd, Spm) and their three different forms in roots and leaves were significantly higher in the Ca 2+ sufficient-hypoxia stress than in the hypoxia stress treatment, but DAO and PAO activity and H_2O_2 contents were lower. In the Ca 2+ deficient treatment, seedlings incurred the greatest damage due to hypoxia stress, and PAs metabolism indices under Ca 2+ deficiency showed opposite trends as compared to seedlings in the Ca 2+ sufficient treatment. We suggest that Ca 2+ might be involved in the regulation of polyamines metabolism and play an important role in enhancing hypoxia tolerance in muskmelon seedlings.
出处
《植物生态学报》
CAS
CSCD
北大核心
2005年第4期652-658,共7页
Chinese Journal of Plant Ecology
基金
国家自然科学基金(30170645)
江苏省自然科学基金(BK2002110)
南京市科技招标项目(2003ZB0201)
关键词
低氧胁迫
钙
网纹甜瓜
幼苗
多胺
多胺氧化酶
酶活性
Calcium, Hypoxia stress, Muskmelon (Cucumis melo var. raticulalus), Polyamines, Polyamine oxidase, H_2O_2
