摘要
成纤维细胞的成骨作用已在体内外得到证实。为进一步探索诱导成纤维细胞成骨潜能得以表现的调控因素，以实现体外获取大量成骨型成纤维细胞、形成骨修复材料的设想，采用分离、纯化人皮肤成纤维细胞，使其生长在含不同浓度的ＥＧＦ、ＩＬ６、ＴＮＦα、ＢＭＰ２培养液的干预条件下，采用生物化学、组织化学和电镜观察方法，检测成纤维细胞成骨性标志物形成状况。发现：ＴＮＦα和ＢＭＰ２联合应用，可使成纤维细胞分泌碱性磷酸酶、骨钙素及胶原的量增加；成纤维细胞由梭形向圆形或多突形转化，蛋白分泌旺盛；细胞外基质中，丰富的胶原纤丝定向或杂乱排列，其间散在较多的钙颗粒；成纤维细胞可重叠交织形成多层结构，其表面分泌颗粒和钙盐结晶堆积，并不断融合扩大成骨结节。认为。
In order to explore further the regulatory factors to the potentiality in inducing osteogenesis by fibroblasts, the fibroblasts were isolated, and purified from human skin, and were grown in incubation in the media of EGF, IL6, TNFα and BMP2 at different concentrations for two weeks, then, the markers for osteogenic features were investigated by biochemistry, histochemistry and electron microscopic observations. It was found that the combined use of TNFα and BMP2 could stimulate fibroblasts to secrete alkaline phosphatase, osteocalcin and collagen, and the morphological changes of the fibroblasts were also very stroking. In the extracellular matrix, the collagen fibrils, with or without periodicity, were arranged regularly or randomly oriented, and numerous minute calcium granules were interspersed among them. The fibroblasts were interwoven one on top of another in the form of multilayer structure and on the surface, there were secreting granules and piling up of calcium crystals which coalessed steadily and increased in size in forming bony nodules. It was considered that TNFα and BMP2 were capable of inducing the fibroblasts to form bone.
出处
《中国修复重建外科杂志》
CAS
CSCD
1998年第4期236-240,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金
