摘要
目的研究联合使用骨形态发生蛋白(rhBMP-2)和碱性成纤维细胞生长因子(bFGF)对成纤维细胞成骨表型的影响。方法向培养的成纤维细胞NIH3T3中加入不同浓度的rhBMP-2和bFGF,观察成纤维细胞形态学变化、碱性磷酸酶(ALP)活性与染色的情况和骨钙素表达的变化,同时观察细胞增殖周期的变化。结果在两种因子作用下,成纤维细胞由长梭形向多角形方向转化,细胞体积增大、细胞内颗粒增多;成纤维细胞ALP活性增高、骨钙素表达增多,细胞内ALP染色加深;细胞增殖周期缩短。结论联合使用rhBMP-2和bFGF可以促进成纤维细胞成骨表型表达并促进细胞增殖。
Objective To evaluate the effect on the osteoge nic phenotype of fibroblasts by usin g rhBMP-2and bFGF togeth-er.Method Add rhBMP-2and bFGF with different c oncentration into the cultured NIH3T3cells.Observe the morphologic ch ange of the cells and the variety of ALP activ ity and the expression of osteocalcin.The staining of ALP in the cells and the proliferation cycle were also under analysis.Result Under the effect of bFGF and rhBMP-2,fibroblast became lager with more pr ocesses and granules.The ALP activity and the expression of osteocalcin increased.The ALP granul es in the cells stained thicker and th e cells' proliferation cycle shortened.Conclusion Using bFGF and rhBMP-2together coul d augment the expression of osteogen ic phenotype and promote prolifer-ation of fibroblasts.
出处
《中国临床康复》
CSCD
2002年第4期498-499,共2页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目(39870792)
