摘要
以PCR扩增新城疫病毒JZ05株的F基因,并进行克隆和测序。采用DNAssist序列分析软件将JZ05毒株F基因与另外18个NDV毒株F基因序列及其氨基酸序列进行比对分析。测序结果表明,NDVJZ05株F基因产物F0蛋白酶裂解位点(111～117位)的氨基酸序列为GRRQKRF。与18个NDV毒株F蛋白的氨基酸序列对比,半胱氨酸残基数目和位置完全一致;仅NDV JZ05株发生单个氨基酸残基变更的有5处;而其F蛋白细胞融合活性位点中的一些保守氨基酸残基都没有发生变更。10个毒株(Ch98、Ch2000、YN、JZ05、JS01、GD05、SCL03、SSX03、Lye01、TW)之间全长氨基酸序列的同源性均在96%以上,这10个毒株与3个疫苗毒株(LaSota、B1、Clone30)的同源性在88.07%～89.51%。
The F gene of Newcastle disease virus isolate JZ05 was amplified with reverse transcription-polymerase chain reaction（RT-PCR）, its gene cloning was completed, and its nucleotide sequence was determined. Comparative analyses on F gene and its amino acids sequence between NDV isolate JZ05 and other 18 NDV isolates was executed using the sequence analysis soft （namely DNAssist）. The F gene sequence of NDV isolate JZ05 showed that amino acids sequence of F0 proteolytic cleavage position （111aa-117aa） was GRRQKRF. Compared with the amino acids sequence of F gene of other 18 NDV isolates,the numbers and positions of cysteine residue were the same. Single amino acid residue varied at five positions just in fusion protein sequence of NDV isolate JZOS,but some conservative amino acid residues had no variation at the active site of cell fusion in fusion protein sequence of NDV isolate JZ05. There were more than 96% identical amino acid residues in fusion protein sequence of 10 NDV isolates （Ch98,Ch2000,YN,JZ05,JS01,GD05,SCL03,SSX03,Lye01,TW）, but the homology of the 10 NDV isolates and 3 vaccine strains （LaSota,B1 ,Clone30）was 88.07%-89.51%.
出处
《湖北农业科学》
北大核心
2009年第2期267-271,共5页
Hubei Agricultural Sciences
基金
湖北省教育厅科学研究计划项目(D200612005)
关键词
新城疫病毒
F基因
基因克隆
序列分析
Newcastle disease virus
F gene
cloning
sequence analysis
