摘要
利用一步法RT -PCR技术成功扩增了 37个新城疫病毒分离株的 (其中 2株属于鸽源病毒 )F基因片段 (约5 0 0bp) ,通过对分离株的测序和基因分型表明 ,2 4株属于基因VII型 ,1株属于基因VI型 ,2株属于基因III型 ,1株属于基因I型 ,6株属于基因II型 ,另外有 3株从氨基酸方面分析是基因VII和VI型的杂交体 ,但从进化树方面 ,分离毒SD0 5 4和SD0 6 9属于基因VI型 ,SD0 5 2属于基因VII型。可以看出 ,我国新城疫的流行是极其复杂的 ,既有老基因型的威胁 ,又有新基因型的不断流行 ,同时发现有
The amplify important function region of the fusion gene of 37 isolates of Newcastle Disease virus was employed to by One step RT-PCR The deduced Amino-acid sequence analysis and The genotypes characteristic showed that 24 strains belong to genotype VII; 2 strains belong to genotype III;one strain belongs to genotype VI; one strain belongs to genotype I; 6 strains belong to genotype II ;other three strains are hybrid of genotype VII and genotype VI through the analysis of the deduced Aminoacid sequences,but from the Phylogenetic tree,SD054 and SD069 belong to genotype VI,SD052 belong to genotype VIIWe can see,the outbreak of Newcastle Disease was very complex in China, that is, not only with the old genotype,but also with the new genotypeMeanwhile, This rpeport found that three strains was the hybrid of genotype VII and VI
出处
《黑龙江畜牧兽医》
CAS
北大核心
2004年第9期10-12,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
农业部新城疫专项基金资助项目
关键词
新城疫病毒
分离株
分子生物学
基因型
新城疫
Newcastle virus(NDV)
one step RT-PCR
genotype VII
genotype VI
hybrid
