摘要
在色谱柱为NucleosilC18反相柱（250mm×4mm），流动相A为体积分数20％的甲醇，流动相B为体积分数30％的乙腈，A和B流动相用体积分数50％的磷酸调pH至3．2±0．05，流动相C为乙腈，三元梯度洗脱，流速0．8～1mL/min，柱温35℃，分析波长272nm条件下，应用反相高效液相色谱法测定了广东产15个品种青果鲜果肉中的没食子酸含量．结果表明，没食子酸线性范围为0．3906—25．0000μg／mL，标准曲线y=62．662x-16．805，相关系数殿=0．9996．标准品保留时间和峰面积的相对标准偏差分别为1．7614％和2．7736％．标准品回收率平均数为99．09％（n=5），回收率相对标准偏差为3．298％．15个品种青果果肉中均含有没食子酸，含量在57．9584～230．7373μg／g鲜质量之间，以土纳甜种品种的含量最高，香种含量最少，相对标准偏差为0．060％～1．938％．
A reverse-phase high-performance liquid chromatography （RP-HPLC） method was used for measuring gallic acid content in flesh of 15 Chinese olives varieties. Samples were injected onto reversedphase Nucleosil C18 column （250 mm ×4 mm i. d. ）. Mobile phase A and B were 20% methanol and 30% aeetonitrile respectively. Both of them were adjusted to pH3.2 with 50% phosphoric acid. Acetoni- trile was set as mobile C. Triple gradient elution proceeded at the flow rate 0. 8-1 mI./min. Operation conditions were as follows： column temperature 35 ℃, detection at 280 nm of wavelength. The results showed that linear calibration of gallic acid was within 0. 390 6-25.000 0 μg/mL with correlation （r） of 0. 999 6. Regression equation was acquired as y =62. 662x-16. 805. Relative standard deviation （RSD） of retained time and peak area of standard sample were respectively 1. 761 4% and 2. 773 6%. Average recovery of standard sample was 99.09% （n =5） with RSD of 3.298%. Gallic acid was detected in all olive samples with the content within 57.958 4 -230. 737 3 μg/g fresh mass （FM）. According to the content of gallic acid, the richest variety was Tunatianzhong while the least was Xiangzhong with RSD of 0. 060%-1. 938% .
出处
《仲恺农业工程学院学报》
CAS
2009年第1期17-19,32,共4页
Journal of Zhongkai University of Agriculture and Engineering
基金
广东省科技计划农业攻关(2006B20201045)资助项目
