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北京地区网织红细胞相关指标正常参考值调查 被引量:4

Investigation and evaluation of reference value of reticulocyte in Beijing
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摘要 摘要:目的:建立北京地区健康成年人群网织红细胞六项指标参数正常值范围。方法:使用Sysmex XT-2000i全自动血细胞分析仪,对207名正常成年人(男性108例年龄23~59岁;女性99例年龄19~55岁)进行网织红细胞相关六项指标调查,将所得数据进行统计学分析得出六项指标参考值范围,并与手工血膜涂片计数法进行对照。结果:RET%:男性1.31±0.34,女性1.19±0.32,两组无显著性差异(P〉0.05)合并后207名RET%为1.25±0.36(0.53-1.97);RET#(×1012/L)为:男性0.67±0.18(0.33-1.01),女性0.54±0.16(0.22-0.86),两组间差异显著(P〈0.05);IRF:男性5.17±2.31(1.41-10.65),女性4.06±1.59(0.88-7.24),两组差异显著(P〈0.05);LRF和MRF的网织红百分数在男女之间有显著差异(P〈0.05);HRF两组无显著性差异(P〉0.05)。挑选20例患者血标本分别用XT-2000i测定与血涂片经亚甲蓝染色手工油镜下计数Ret结果进行比较,结果P〉0.05,两法测定结果的差异无统计学意义。结论:成人网织红细胞六项指标参数正常值范围的测定因为地区、人种、性别、仪器等因素影响而不同,因此,不同地区各实验室应根据本地区人群建立自己的正常参考范围,这样网织红细胞参数才能更好地为临床所应用。同时使用仪器法与手工法测定比较两者的相关性较好,说明仪器法能取代手工法应用于临床。 Objective:To establish the reference range of 6 reticulocyte parameters of Beijing.Methods:With Sysmex XT-2000i,we randomly studied 207 cases of healthy adults(108 cases of male,female 99 cases) on reference ranges of reticulocyte parameters.At the same time,the classification produced by the XT-2000i and manual methods were compared.Results:RET%:male is 1.31±0.34,female is 1.19±0.32,there was no significant between each age groups(P〉0.05),unite RET% 1.25±0.36(0.53-1.97);RET#(×1012/L):male is 0.67±0.18(0.33-1.01),female is 0.54±0.16(0.22-0.86)(P〈0.05);IRF;male is 5.17±2.31(1.41-10.65),female is 4.06±1.59(0.88-7.24),there was significant difference between each age groups(P〈0.05);on LRF,MRF and HRF,there was significant difference between each age groups on LRF and MRF(P〈0.05),except HRF(P〉0.05).And compared with the manual methods:(P〉0.05).Conclusion:There is no significant difference between reference ranges of 6 reticulocyct parameters with some references.But area,race,sex,analyzer and so on are correlated with the counting results of reticulocyte parameters,so the establishment of this reference range is necessary by laboratory itself for the operation of laboratory and clinic study.At the same time,Compared XT-2000i with the manual methods,the relativity is better.It can replace the manual methods.
出处 《中国卫生检验杂志》 CAS 2009年第3期700-701,共2页 Chinese Journal of Health Laboratory Technology
关键词 网织红细胞 血细胞分析仪 正常参考值 调查 Reticulocyte Automated hematology analyzer Reference range Investigation
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