摘要
目的：观察自发性高血压大鼠（ＳＨＲ）心肌组织细胞膜内皮素受体的变化。方法：４个月ＳＨＲ８只为实验组，４个月Ｗｉｓｔａｒ－Ｋｙｏｔｏ（ＷＫＹ）大鼠８只为对照组。采用放射性配基受体结合分析法，从蛋白水平测定心肌组织细胞膜内皮素受体的含量。结果：ＳＨＲ及ＷＫＹ大鼠心肌组织细胞膜１２５Ｉ－ＥＴ－１最大结合位点（Ｂｍａｘ）分别为（１３６．５７±１５．３８）和（１６１．７４±１８．９８）ｆｍｏｌ／ｍｇｐｒｏ（Ｐ＜０．０５）；平衡解离常数分别为（１５９．５４±１８．３１）和（１８０．９７±２８．６２）ｐｍｏｌ／Ｌ（Ｐ＞０．０５）；Ｈｉｌ系数分别为０．９８６±０．００６和０．９８０±０．００７（Ｐ＜０．０５）。结论：ＳＨＲ和ＷＫＹ大鼠心肌含有特异的高亲和力及可饱和的１２５Ｉ－ＥＴ－１的结合位点，而且受体间无协同性，其中ＳＨＲ心肌１２５Ｉ－ＥＴ－１的Ｂｍａｘ明显低于ＷＫＹ大鼠。
Objective: To observe the changes of endothelin receptor in the heart of spontaneously hypertensive rats (SHRs).Methods:Four months old rats were divided into two groups: SHRs (n=8) and WKY rats (n=8). 125IET1 binding sites, affinity, and Hill coefficient in the cardiac membranes from left ventricle were examined by radio ligand binding study.Results:125IET1 binding site density in left ventricular membranes from SHRs was reduced (P<0.05) as compared with that of WKY rats. There were no marked changes for KD and Hill coefficient between these two groups.Conclusion:These results suggest there are specific, high affinity, and a single population of endothlin1 binding sites in left ventricular membranes from SHRs and WKY rats. In particular,125IET1 binding site density in left ventricle of SHRs is significantly lower than that of WKY rats.
出处
《军医进修学院学报》
CAS
1998年第2期104-106,共3页
Academic Journal of Pla Postgraduate Medical School
基金
"八五"军队医药卫生科研基金
关键词
高血压
心肌
内皮素受体
hypertenson
rats
myocardium
receptor, endothelin
