摘要
目的采用窝沟封闭体外实验龋模型,研究在唾液污染的情况下,使用全酸蚀粘接剂Prime&BondNT对窝沟封闭后继发类龋损发生和发展的影响,探讨使用粘接剂提高窝沟封闭防龋效果的可行性。方法选择无龋正畸减数上前磨牙36颗,随机分为3组(12颗/组):常规组、唾液污染组、唾液污染+全酸蚀粘接剂组(Prime&Bond NT),分别采用不同的方法处理后进行窝沟封闭,各组标本经继发类龋损致龋实验后,应用激光共聚焦扫描显微镜观测各组标本窝沟封闭剂下龋损荧光面积,总荧光量,平均荧光量,封闭剂下龋损最大深度。结果唾液污染组上述各参数均显著高于常规组(P<0.01),唾液污染+全酸蚀粘接剂组(Prime&Bond NT)各参数与常规组比较无显著差异(P>0.05)。结论唾液污染牙面会导致窝沟封闭后继发龋损的产生,影响封闭效果。使用全酸蚀粘接剂(Prime&Bond NT)可以减少唾液污染产生的不良影响,抑制窝沟封闭后继发龋损的发展,提高窝沟封闭防龋效果。
Objective To study the caries - preventive effect of bonding agent （Prime ＆ Bond NT） before placing fissure sealant under the condition of saliva contamination. Methods Thirty six caries - free maxillary premolars （extracted for orthodontic treatments） were randomly divided into three groups of 12 teeth each. Group 1 was treated with fissure sealant routinely （sealed with a resin based fissure sealant after phosphoric acid etching ）. Group 2 was slightly contaminated with saliva before routine sealant application. Group 3 was sealed with a total -etching adhesive （Prime ＆ Bond NT） after saliva contamination. After 100 thermo - cycling, the specimens were exposed to an in vitro microbial - based model to study the secondary caries - like lesions under fissure sealants. The degree of demineralization was assessed by Confocal Laser Scanning Microscope （CLSM） to measure four lesion parameters （area of fluorescence, total and average value of fluorescence, maximum depth of the lesion）. The data were collected and analyzed with one - way ANOVA. Results The four lesion parameters of group 2 was significantly higher than those of group 1 （ P 〈 0.01 ）. There was no significant difference regarding the extent of secondary caries - like lesion between group 1 and group 3 （ P 〉 0.05 ）. Conclusion Saliva - contamination has negative effect on the fissure sealants. Total - etching adhesive （ Prime ＆ Bond NT） can improve caries - preventive effect of fissure sealing under the condition of saliva contamination.
出处
《现代口腔医学杂志》
CAS
CSCD
2009年第2期195-198,共4页
Journal of Modern Stomatology
关键词
窝沟龋
窝沟封闭
继发类龋损
Pit and fissure caries Fissure sealant Secondary caries -like lesion
